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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
STEM_545_sm_suppinfofigure1.tif109KSupplement Figure 1: The experimental setup used for the optical mapping studies.
STEM_545_sm_suppinfofigure2.tif330KSupplement Figure 2: Formation of myotubes (ex-vivo) by the skeletal myoblasts. [A] Initial immonostaining with myogenin antibodies (green) showing the cultured skeletal myoblasts prior to the formation of myotubes. [B-C] Formation of myotubes as identified by phase contrast microscopy (B) and nuclear immunostaining for myogenin (C- left) and myogenin and DAPI (C-right).
STEM_545_sm_suppinfofigure3.tif64KSupplement Figure 3: Low magnification fluorescent image of the skeletal-myocyte transplanted area within the rat myocardium used for graft-size quantification. The transplanted cells were pre-labeled with the fluorescent cell tracker Q-tracker 585.
STEM_545_sm_suppinfomovie1.wmv2465KSupplement Movie 1: Dynamic display showing ventricular activation spread in a myoblast-transplanted heart. Notice the presence of localized conduction block at the site of cell grafting.
STEM_545_sm_suppinfomovie2.wmv2561KSupplement Movie 2: Dynamic display showing ventricular activation spread in a myoblast-transplanted heart. Notice the presence of localized conduction slowing at the site of cell grafting.
STEM_545_sm_suppinfomovie3.wmv5121KSupplement Movie 3: Optical mapping movie displaying a ventricular tachycardia circuit in a myoblast-transplanted heart. Notice the reentrant activation wavefront circulating around the site of cell grafting.
STEM_545_sm_suppinfomovie4.wmv3137KSupplement Movie 4: Dynamic display showing ventricular activation spread in a hESC-CMs-engrafted heart. Notice the absence of any conduction disturbances at the site of cell grafting.
STEM_545_sm_suppinfomovie5.wmv6209KSupplement movie 5: Dynamic display showing ventricular activation spread in a hESC-CMs-transplanted heart. Notice the presence of slight conduction slowing at the site of cell grafting.

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