Concise Review

General characterization of human cytomegalovirus-specific proliferative cd4+t cell clones

  1. Yung-Nan C. Liu1,*,
  2. June Eckhardt1,
  3. Bruce Kari1,
  4. Richard C. Gehrz2

Article first published online: 1 JAN 1996

DOI: 10.1002/stem.5530060506

Issue

The International Journal of Cell Cloning

The International Journal of Cell Cloning

Volume 6, Issue 5, pages 352–364, 1988

Additional Information

How to Cite

Liu, Y.-N. C., Eckhardt, J., Kari, B. and Gehrz, R. C. (1988), General characterization of human cytomegalovirus-specific proliferative cd4+t cell clones. The International Journal of Cell Cloning, 6: 352–364. doi: 10.1002/stem.5530060506

Author Information

  1. 1

    Sutton Immunobiology Research Laboratory, Biomedical Research Institute, Children's Hospital of St. Paul, St. Paul, Minnesota, USA;

  2. 2

    The Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA

*Biomedical Research Institute, Children's Hospital of St. Paul, St. Paul MN 55102, USA

Publication History

  1. Issue published online: 2 JAN 2009
  2. Article first published online: 1 JAN 1996
  3. Manuscript Accepted: 15 JUN 1988
  4. Manuscript Received: 18 MAY 1988

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Keywords:

  • Cytomegalovirus;
  • Cell-mediated immunity;
  • T helper cells;
  • T cell clones

Abstract

Over 80 human T helper cell (Th) clones reactive with human cytomegalovirus (HCMV) were generated using purified whole Towne strain HCMV as the in vitro antigen. These cloned T cells are CD3+, CD4+, CD8- and proliferate specifically to HCMV. All of the clones tested produce interleukin 2 and gamma-interferon and failed to show HCMV-specific cytotoxicity or natural killer (NK) activity. Most of the Th clones recognize multiple laboratory-adapted and wild-type strains of HCMV. The Th clones were also tested for their reactivity to a major envelope glycoprotein complex (gcl) and a 64,000 dalton internal matrix protein. Our results show that both proteins as well as other unidentified protein(s) are involved in Th responses to HCMV.

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