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STEM_566_sm_suppinfofigure1.eps14406KSupplemental figure 1. The 3T3 E1 murine osteogenic precursor line was used as a control for alizarin red and von Kossa histochemical stains. 3T3E1 cells were cultured in DMEM with 10% FBS or osteogenic differentiation medium. The specificity of staining was confirmed by using the respective negative (A,B) and positive (C,D) controls. The images were taken with 10x objective.
STEM_566_sm_suppinfofigure2.tif1004KSupplemental figure 2. Controls for semiquantitative PCR included RNA isolated from the 3T3 E1 osteogenic precursor line cultured in differentiation medium (diff bone) and whole bone marrow. The specificity of runx2, col1a1 and spp1 gene analysis was confirmed by semiquantiative PCR using the respective positive and negative controls.
STEM_566_sm_suppinfofigure3.tif1476KSupplemental figure 3. A contextual perspective of phenotypic analysis of isolated osteoblast seeded scaffolds. After 12 weeks, gelfoam scaffolds were embedded in OCT, frozen and 25μM sections analyzed. Consecutive sections were immunoreactive to osteocalcin and bone sialoprotein (B-C). DAPI was used to label nuclei (A). Scale bar = 2.5mm.

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