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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 24 FEB 2011
Copyright © 2010 AlphaMed Press
Volume 29, Issue 2, pages 206–216, February 2011
How to Cite
Bilousova, G., Jun, D. H., King, K. B., De Langhe, S., Chick, W. S., Torchia, E. C., Chow, K. S., Klemm, D. J., Roop, D. R. and Majka, S. M. (2011), Osteoblasts Derived from Induced Pluripotent Stem Cells form Calcified Structures in Scaffolds Both In Vitro and In Vivo. STEM CELLS, 29: 206–216. doi: 10.1002/stem.566
G.B.: conception and design, collection and/or assembly of data, provision of study material or patients data analysis and interpretation, manuscript writing; D.H.J.: conception and design, collection and/or assembly of data, data analysis and interpretation; K.B.K.: provision of study material or patients, data analysis and interpretation, final approval of manuscript; S.D.L.: collection and/or assembly of data; W.S.C.: conception and design; E.C.T.: collection and/or assembly of data, data analysis and interpretation; K.S.C.: collection and/or assembly of data, data analysis and interpretation; D.J.K.: collection and/or assembly of data, data analysis and interpretation; D.R.R.: financial support, provision of study material or patients; S.M.M.: conception and design, financial support, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript. G.B. and D.H.J. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 23, 2010.
- Issue published online: 24 FEB 2011
- Article first published online: 24 FEB 2011
- Accepted manuscript online: 23 NOV 2010 07:30AM EST
- Manuscript Accepted: 9 NOV 2010
- Manuscript Received: 3 AUG 2010
- Unknown funding agency. Grant Numbers: R03HL096382-01, 1R01 HL091105-01
- NIH. Grant Numbers: AR052263, AR50252
- Dystrophic Epidermolysis Bullosa Research Association (DebRA) International from DebRA Austria
- DK NIDDK. Grant Number: R01-078966
Additional supporting information available online.
|STEM_566_sm_suppinfofigure1.eps||14406K||Supplemental figure 1. The 3T3 E1 murine osteogenic precursor line was used as a control for alizarin red and von Kossa histochemical stains. 3T3E1 cells were cultured in DMEM with 10% FBS or osteogenic differentiation medium. The specificity of staining was confirmed by using the respective negative (A,B) and positive (C,D) controls. The images were taken with 10x objective.|
|STEM_566_sm_suppinfofigure2.tif||1004K||Supplemental figure 2. Controls for semiquantitative PCR included RNA isolated from the 3T3 E1 osteogenic precursor line cultured in differentiation medium (diff bone) and whole bone marrow. The specificity of runx2, col1a1 and spp1 gene analysis was confirmed by semiquantiative PCR using the respective positive and negative controls.|
|STEM_566_sm_suppinfofigure3.tif||1476K||Supplemental figure 3. A contextual perspective of phenotypic analysis of isolated osteoblast seeded scaffolds. After 12 weeks, gelfoam scaffolds were embedded in OCT, frozen and 25μM sections analyzed. Consecutive sections were immunoreactive to osteocalcin and bone sialoprotein (B-C). DAPI was used to label nuclei (A). Scale bar = 2.5mm.|
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