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Tissue-Specific Stem Cells
Version of Record online: 24 FEB 2011
Copyright © 2010 AlphaMed Press
Volume 29, Issue 2, pages 332–343, February 2011
How to Cite
Leong, S. Y., Faux, C. H., Turbic, A., Dixon, K. J. and Turnley, A. M. (2011), The Rho Kinase Pathway Regulates Mouse Adult Neural Precursor Cell Migration. STEM CELLS, 29: 332–343. doi: 10.1002/stem.577
Author contributions: S.Y.L.: conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing; C.H.F. and A.T.: collection and assembly of data, data analysis and interpretation; K.J.D.: collection of data; A.M.T.: conception and design, financial support, data analysis and interpretation, manuscript writing, final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS December 9, 2010.
- Issue online: 24 FEB 2011
- Version of Record online: 24 FEB 2011
- Accepted manuscript online: 9 DEC 2010 08:27AM EST
- Manuscript Accepted: 19 NOV 2010
- Manuscript Received: 2 FEB 2010
- National Health and Medical Research Council of Australia Project grant. Grant Numbers: 454384, 350226
Additional supporting information available online.
|STEM_577_sm_suppinfoFig1.tif||459K||Supplementary Fig. 1. Western blot analysis of ROCK, Rac1 and PI3K inhibition on the activation of Akt at 24hrs. (A) Lysates derived from NPCs treated with Y27632 (Y), Rac1 inhibitor and LY294002 (LY) were assessed by Western blotting for phosphorylated and total levels of Akt. (B) Densitometric analysis comparing the ratio of phosphorylated Akt to total Akt. Mean ± SEM of n=3 independent experiments. **P<0.01; ***P<0.001.|
|STEM_577_sm_suppinfoFig2.tif||474K||Supplementary Fig. 2. Western blot analysis of ROCK, Rac1 and PI3K inhibition on the activation of myosin light chain II at 24hrs. (A) Lysates derived from NPCs treated with Y27632 (Y), Rac1 inhibitor and LY294002 (LY) were assessed by Western blotting for phosphorylated and total levels of myosin light chain II (MLCII). (B) Densitometric analysis comparing the ratio of phosphorylated MLCII to total MLCII. Mean ± SEM of n=3 independent experiments. **P<0.01; ***P<0.001.|
|STEM_577_sm_suppinfoFig3.tif||1157K||Supp. Fig. 3. Effect of ROCK inhibition at the cortical injury site. (A, C, D) Infusion of BrdU+/-Y27632 into the lateral ventricle produced a stab injury in the right cortex (right of panel A). Immunohistochemistry for BrdU (red) and GFAP (green), showed numerous BrdU+ and GFAP+ cells, especially in the area close to the margin of the injury at 1 week. White arrow in (A) indicates midline. LV, lateral ventricle; IS injury site. Scale bars; (A) 200μm, (C, D) 50μm. ROCK inhibition did not affect the total number of BrdU+ cells at (B) the injury periphery (represented by boxes 1-4 in panel A) or the injury margin (represented by columns in panel A). (E) ROCK inhibition increased the percentage of proliferative astrocytes at the injury margin but had no effect on CD11b+ macrophages. Mean ± SEM of 5 treated mice and 4 control mice. *P<0.05.|
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