Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 12 MAR 2009
Copyright © 2009 AlphaMed Press
Volume 27, Issue 6, pages 1265–1275, June 2009
How to Cite
Wang, X., Zhao, Y., Xiao, Z., Chen, B., Wei, Z., Wang, B., Zhang, J., Han, J., Gao, Y., Li, L., Zhao, H., Zhao, W., Lin, H. and Dai, J. (2009), Alternative Translation of OCT4 by an Internal Ribosome Entry Site and its Novel Function in Stress Response. STEM CELLS, 27: 1265–1275. doi: 10.1002/stem.58
Author contributions: X.W.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing; Y.Z.: provision of study material or patients, data analysis and interpretation; Z.X.: collection and/or assembly of data, data analysis and interpretation; B.C.: provision of study material or patients, data analysis and interpretation; Z.W.: collection and/or assembly of data; B.W.: data analysis and interpretation; J.Z.: data analysis and interpretation; J.H.: data analysis and interpretation; Y.G.: data analysis and interpretation; L.L.: provision of study material or patients; H.Z.: provision of study material or patients; W.Z.: data analysis and interpretation; H.L.: data analysis and interpretation; J.D.: conception and design, financial support, data analysis and interpretation, final approval of manuscript.
First published online in STEM CELLSExpress March 12, 2009
- Issue published online: 1 JUN 2009
- Article first published online: 12 MAR 2009
- Accepted manuscript online: 12 MAR 2009 12:00AM EST
- Manuscript Accepted: 3 MAR 2009
- Manuscript Received: 12 AUG 2008
- Ministry of Science and Technology of China. Grant Number: 2006CB943601
- NSFC. Grant Numbers: 30688002, 30600304, 30800564
- Chinese Academy of Sciences. Grant Number: KSCX2-YW-R-133
Additional supporting information available online.
|STEM_58_sm_suppinfo.doc||45K||Supporting Information and Methods|
|STEM_58_sm_suppinfofigure1.tif||418K||Supporting Information Figure 1. Analysis of endogenous OCT4B-190 protein expression under heat shock. (A) MCF-7 cells were treated with heat shock at 45° (lanes 1-5). (B) Hela cells were treated with heat shock at 45° (lanes 1-5). Each experiment was repeated three times.|
|STEM_58_sm_suppinfofigure2.tif||978K||Supporting Information Figure 2. Determination of the methylation status of the diatal enhancer region of the OCT4 promoter. The distal enhancer region of OCT4 promoter is unmethylated in PA-1 cells and relative highly methylated in HepG2, Hela, OS732, MCF-7, HEK293 and fibroblast cells.|
|STEM_58_sm_suppinfofigure3.tif||327K||Supporting Information Figure 3. Determination of the promoter activity of the putative IRES element of OCT4B. 1: PGL3-Basic vector was used as a negative control; 2: PGL3-Promoter vector was used as a positive control; 3: PGL3-OCT4B (IRES) contained the putative IRES element of OCT4B which inserted into the PGL3-Basic vector. PGL3-Basic vector, PGL3-Promoter vector and PGL3-OCT4B (IRES) vector were respectively cotransfected with pRL-TK vector in MCF-7 cells. pRL-TK vector expressing the Renilla luciferase was used as a normalization.|
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