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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 21 MAR 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 3, pages 496–504, March 2011
How to Cite
Peng, S., Chen, L.-L., Lei, X.-X., Yang, L., Lin, H., Carmichael, G. G. and Huang, Y. (2011), Genome-Wide Studies Reveal That Lin28 Enhances the Translation of Genes Important for Growth and Survival of Human Embryonic Stem Cells. STEM CELLS, 29: 496–504. doi: 10.1002/stem.591
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: S.P.: collection and assembly of data, data analysis and interpretation; L.-L.C.: data analysis and interpretation, financial support; X.-X.L.: collection and assembly of data; L.Y.: data analysis and interpretation; H.L.: conception and design, financial support; G.G.C.: conception and design, financial support; Y.H.: conception and design, data analysis and interpretation, financial support.
First published online in STEM CELLSEXPRESS January 7, 2011.
- Issue published online: 21 MAR 2011
- Article first published online: 21 MAR 2011
- Accepted manuscript online: 7 JAN 2011 03:49PM EST
- Manuscript Accepted: 16 DEC 2010
- Manuscript Received: 1 NOV 2010
- the Connecticut Stem Cell Research. Grant Numbers: grants 09SCAYALE14, 06SCB08, 09SCAUCHC16, 06SCD01
- Stem cell;
Lin28 inhibits the expression of let-7 microRNAs but also exhibits let-7-independent functions. Using immunoprecipitation and deep sequencing, we show here that Lin28 preferentially associates with a small subset of cellular mRNAs. Of particular interest are those for ribosomal proteins and metabolic enzymes, the expression levels of which are known to be coupled to cell growth and survival. Polysome profiling and reporter analyses suggest that Lin28 stimulates the translation of many or most of these targets. Moreover, Lin28-responsive elements were found within the coding regions of all target genes tested. Finally, a mutant Lin28 that still binds RNA but fails to interact with RNA helicase A (RHA), acts as a dominant-negative inhibitor of Lin28-dependent stimulation of translation. We suggest that Lin28, working in concert with RHA, enhances the translation of genes important for the growth and survival of human embryonic stem cells. STEM CELLS 2011;496–504