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Tissue-Specific Stem Cells
Article first published online: 21 MAR 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 3, pages 539–548, March 2011
How to Cite
Lee, D., Kim, T. and Lim, D.-S. (2011), The Er71 Is an Important Regulator of Hematopoietic Stem Cells in Adult Mice. STEM CELLS, 29: 539–548. doi: 10.1002/stem.597
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: D.L., collection and assembly of data, manuscript writing; T.K., technical support; D.-S.L., adviser, conception and design, manuscript writing.
First published online in STEM CELLSEXPRESS December 29, 2010.
- Issue published online: 21 MAR 2011
- Article first published online: 21 MAR 2011
- Accepted manuscript online: 14 JAN 2011 12:27PM EST
- Manuscript Accepted: 29 DEC 2010
- Manuscript Received: 13 OCT 2010
- the National Creative Research Initiatives Program. Grant Number: 2010-0018277
- World Class University Program of the National Research Foundation of Korea (NRF)
- the Ministry of Education, Science and Technology. Grant Number: R31-2008-000-10071-0
- Hematopoietic stem cells;
- Progenitor cells;
- Conditional knockout mouse;
- Tie2 and ER71
The Ets transcription factor Er71 is an important regulator of endothelial and hematopoietic development during mammalian embryogenesis. However, the role of Er71 in adult hematopoiesis has remained unknown. We now first show that conditional deletion of Er71 in the hematopoietic system of adult mice results in a marked reduction (55%) in the number of hematopoietic stem cells (HSCs) that is likely due to increased cell death. Bone marrow transplantation (BMT) experiments further confirmed that Er71 is required for repopulation of HSCs. In addition, Er71+/− mice exhibited a slight decrease (37%) in the number of HSCs than those of Er71+/+ mice, indicating that the function of Er71 in HSC maintenance is dependent on gene dosage. Moreover, Er71 was shown to be required for Tie2 expression, which contributes to HSC maintenance. Our results thus suggest the role of a single transcription factor in controlling HSCs through regulation of Tie2 expression in adult animals. STEM CELLS 2011;29:539–548