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Tissue-Specific Stem Cells
Article first published online: 5 APR 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 4, pages 678–688, April 2011
How to Cite
Shahi, P., Seethammagari, M. R., Valdez, J. M., Xin, L. and Spencer, D. M. (2011), Wnt and Notch Pathways Have Interrelated Opposing Roles on Prostate Progenitor Cell Proliferation and Differentiation. STEM CELLS, 29: 678–688. doi: 10.1002/stem.606
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: P.S.: conception and design, provision of study material, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; M.R.S.: collection and/or assembly of data; J.M.V.: collection and/or assembly of data; L.X.: conception and design, data analysis and interpretation; D.M.S.: conception and design, financial support, provision of study material, data analysis and interpretation, manuscript writing, final approval of manuscript.
First published online in STEM CELLSEXPRESS February 3, 2011.
- Issue published online: 5 APR 2011
- Article first published online: 5 APR 2011
- Accepted manuscript online: 3 FEB 2011 01:14PM EST
- Manuscript Accepted: 8 JAN 2011
- Manuscript Received: 1 JUL 2010
- NIH. Grant Numbers: #T32-AI0749510, UO1-CA141497
- Prostate stem cell;
Tissue stem cells are capable of both self-renewal and differentiation to maintain a constant stem cell population and give rise to the plurality of cells within a tissue. Wnt signaling has been previously identified as a key mediator for the maintenance of tissue stem cells; however, possible cross-regulation with other developmentally critical signaling pathways involved in adult tissue homeostasis, such as Notch, is not well understood. By using an in vitro prostate stem cell colony (“prostasphere”) formation assay and in vivo prostate reconstitution experiments, we demonstrate that Wnt pathway induction on Sca-1+CD49f+ basal/stem cells (B/SCs) promotes expansion of the basal epithelial compartment with noticeable increases in “triple positive” (cytokeratin [CK] 5+, CK8+, p63+) prostate progenitor cells, concomitant with upregulation of known Wnt target genes involved in cell-cycle induction. Moreover, Wnt induction affects expression of epithelial-to-mesenchymal transition signature genes, suggesting a possible mechanism for priming B/SC to act as potential tumor-initiating cells. Interestingly, induction of Wnt signaling in B/SCs results in downregulation of Notch1 transcripts, consistent with its postulated antiproliferative role in prostate cells. In contrast, induction of Notch signaling in prostate progenitors inhibits their proliferation and disrupts prostasphere formation. In vivo prostate reconstitution assays further demonstrate that induction of Notch in B/SCs disrupts proper acini formation in cells expressing the activated Notch1 allele, Notch-1 intracellular domain. These data emphasize the importance of Wnt/Notch cross-regulation in adult stem cell biology and suggest that Wnt signaling controls the proliferation and/or maintenance of epithelial progenitors via modulation of Notch signaling. STEM CELLS 2011;29:678–688