Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells§

Authors

  • Nessy John,

    1. Division of Cell and Developmental Biology, Institute of Anatomy, University of Zurich, Zurich, Switzerland
    2. Division of Biology, Institute of Cell Biology, ETH Zurich, Zurich, Switzerland
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  • Paolo Cinelli,

    1. Institute of Laboratory Animal Science, University of Zurich, Zurich, Switzerland
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  • Michael Wegner,

    1. Institute for Biochemistry, Emil Fischer Center, University of Erlangen-Nuernberg, Germany
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  • Lukas Sommer

    Corresponding author
    1. Division of Cell and Developmental Biology, Institute of Anatomy, University of Zurich, Zurich, Switzerland
    • Division of Cell and Developmental Biology, Institute of Anatomy, University of Zurich, Zurich CH-8057, Switzerland.
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    • Ph: 41-44-635-53-50; Fax: 41-44-635-68-95


  • Disclosure of potential conflicts of interest is found at the end of this article.

  • Author contributions: N.J.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing and final approval of manuscript; P.C.: collection and/or assembly of data and final approval of manuscript; M.W.: provision of study material and final approval of manuscript; L.S.: conception and design, financial support, manuscript writing, and final approval of manuscript.

  • §

    First published online in STEM CELLSEXPRESS February 3, 2011.

Abstract

During vertebrate development, neural crest stem cells (NCSCs) give rise to neural cells of the peripheral nervous system and to a variety of mesenchymal cell types, including smooth muscle, craniofacial chondrocytes, and osteocytes. Consistently, mesenchymal stem cells (MSCs) have recently been shown to derive in part from the neural crest (NC), although the mechanisms underlying MSC generation remains to be identified. Here, we show that transforming growth factor β (TGFβ)-mediated suppression of the NCSC transcription factor Sox10 induces a switch in neural to mesenchymal potential in NCSCs. In vitro and in vivo, TGFβ signal inactivation results in persistent Sox10 expression, decreased cell cycle exit, and perturbed generation of mesenchymal derivatives, which eventually leads to defective morphogenesis. In contrast, TGFβ-mediated downregulation of Sox10 or its genetic inactivation suppresses neural potential, confers mesenchymal potential to NC cells in vitro, and promotes cell cycle exit and precocious mesenchymal differentiation in vivo. Thus, negative regulation of Sox10 by TGFβ signaling promotes the generation of mesenchymal progenitors from NCSCs. Our study might lay the grounds for future applications demanding defined populations of MSCs for regenerative medicine. STEM CELLS 2011;29:689–699

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