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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 5 APR 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 4, pages 609–617, April 2011
How to Cite
Jiang, W., Sui, X., Zhang, D., Liu, M., Ding, M., Shi, Y. and Deng, H. (2011), CD24: A Novel Surface Marker for PDX1-Positive Pancreatic Progenitors Derived from Human Embryonic Stem Cells. STEM CELLS, 29: 609–617. doi: 10.1002/stem.608
Disclosure of potential conflicts of interest is found at the end of this article.
Author contributions: W.J. and X.S.: conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing; D.Z.: collection and assembly of data, data analysis and interpretation; M.L.: collection and assembly of data; M.D.: data analysis and interpretation; Y.S.: manuscript writing, administrative support, final approval of manuscript; H.D.: manuscript writing, financial support, administrative support, final approval of manuscript. W.J. and X.S. contributed equally to this article.
First published online in STEM CELLSEXPRESS February 3, 2011.
- Issue published online: 5 APR 2011
- Article first published online: 5 APR 2011
- Accepted manuscript online: 3 FEB 2011 01:14PM EST
- Manuscript Accepted: 13 JAN 2011
- Manuscript Received: 27 SEP 2010
- National Basic Research Program for China. Grant Numbers: 973, 2009CB941203
- National Natural Science Foundation of China. Grant Number: 30830061
- National Key Technology R&D Program. Grant Number: 2011ZX0 9102-010-03
- Beijing Natural Science Foundation. Grant Number: 5100002
- Pancreatic surface marker;
- Pancreatic progenitors;
- Human embryonic stem cells
Human ESCs provide a promising cell resource for the treatment of type I diabetes mellitus. Although PDX1-positive pancreatic progenitors can be efficiently generated from human ESCs by stepwise induction, further in vitro differentiation into functional, mature beta cells is not efficient or reproducible. Purification of pancreatic progenitor cells could facilitate the identification of signals that regulate beta cell differentiation and maturation. Here, we report the identification of a novel surface marker for PDX1-positive pancreatic progenitors based on an in vitro human ESC differentiation system. By costaining PDX1 and a panel of cell surface antigens at the pancreatic progenitor stage of human ESC differentiation, we discovered a positive marker, CD24. CD24-positive cells coexpressed most of the key transcription factors of pancreatic progenitors, and the expression of important pancreatic genes was greatly enriched in CD24-positive cells compared with the CD24-negative cells. In addition, CD24-positive cells could differentiate into insulin-producing cells but CD24-negative cells could not. These results indicate that CD24 could be a surface marker for PDX1-positive pancreatic progenitors derived from human ESCs. Enrichment of pancreatic progenitors with this marker will facilitate the investigation of beta cell maturation during the human ESC differentiation. STEM Cells 2011;29:609–617