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Keywords:

  • Neural stem cells;
  • p53;
  • Glioma;
  • Microarray;
  • Oligodendrocyte precursor cells;
  • HRas;
  • Glioma-initiating cells

Abstract

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

Recent findings have demonstrated that malignant tumors, including glioblastoma multiforme (GBM), contain cancer-initiating cells (CICs; also known as cancer stem cells), which self-renew and are malignant. However, it remains controversial whether such CICs arise from tissue-specific stem cells, committed precursor cells, or differentiated cells. Here, we sought to examine the origin of the CICs in GBM. We first showed that the overexpression of oncogenic HRasL61 transformed p53-deficient oligodendrocyte precursor cells (OPCs) and neural stem cells (NSCs) into glioma-initiating cell (GIC)-like cells in mice. When as few as 10 of these GIC-like cells were transplanted in vivo, they formed a transplantable GBM with features of human GBM, suggesting that these GIC-like cells were enriched in CICs. DNA microarray analysis showed that widespread genetic reprogramming occurred during the OPCs' transformation: they largely lost their OPC characteristics and acquired NSC ones, including the expression of prominin1, hmga2, ptgs2, and epiregulin. In addition, the combination of a Ptgs2 inhibitor and an epidermal growth factor receptor (EGFR)-signaling inhibitor prevented the tumorigenesis of transformed OPCs and human GICs (hGICs) obtained from anaplastic oligodendroglioma, but not of transformed NSCs or hGICs obtained from GBM. Together, these findings suggest that GBM can arise from either OPCs or NSCs and that the therapeutic targets for GBM might be different, depending on each GIC's cell-of-origin. STEM Cells 2011;29:590–599


INTRODUCTION

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

Cancer-initiating cells (CICs; also referred to as cancer stem cells) self-renew indefinitely and continuously generate the amplifying cancer cells that form the majority of cells in a tumor [1, 2]. It is believed that CICs can arise from normal tissue-specific stem cells, precursor cells, or differentiated cells that have acquired stem cell characteristics as a result of oncogenic mutation. In fact, the overexpression of oncogenes can induce hematopoietic stem/progenitor cells to transform into leukemic stem cell-like cells, which form leukemia when a small number of them are transplanted in vivo [2, 3], suggesting that such induced cancer models can be used to investigate the cell-of-origin for CICs. A number of mutations in oncogenes and tumor-suppressor genes are known to be involved in tumorigenesis; however, except for leukemia, the relationship between the cell-of-origin for CIC and such genetic alterations has not been determined [2, 3].

We and others have shown that specified oligodendrocyte precursor cells (OPCs) and astrocytes can revert to neural stem-like cells that, when cultured under appropriate conditions, can form floating aggregates, express neural stem cell (NSC) markers, and differentiate into neurons as well as glial cells [4–7], suggesting that OPCs and astrocytes, as well as NSCs, can serve as the cell-of-origin for brain CICs.

Among a number of mutations associated with gliomagenesis, p53 is the most frequently mutated tumor-suppressor gene in human glioblastoma multiforme (GBM) [8–11], and increased activation of the Ras-signaling pathway is also found in human gliomas, including about 90% of GBM cases [10–12]. Moreover, the deletion of both p53 and NF1, which negatively regulates the Ras-signaling pathway, causes malignant gliomas in mice [13, 14]. Together, these findings led us to examine whether the combination of p53 deletion and Ras activation can induce cultured NSCs, OPCs, and astrocytes to transform into glioma-initiating cell (GIC)-like cells.

MATERIALS AND METHODS

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

Animals and Chemicals

Mice were obtained from the Laboratory for Animal Resources and Genetic Engineering at the RIKEN Center for Developmental Biology (CDB) and from Charles River Japan, Inc. All mouse experiments were performed following protocols approved by the RIKEN CDB Animal Care and Use Committee. Genotyping of p53-knockout mice was done by polymerase chain reaction (PCR) as described previously [15]. Chemicals and growth factors were purchased from Sigma-Aldrich (St. Louis, MO, www.sigmaaldrich.com) and Peprotech (Rocky Hill, NJ, www.peprotechec.com), respectively, except where indicated.

Cell Culture

NSCs were prepared from embryonic day 13.5 p53-deficient mouse telencephalon and expanded as described previously [16]. For immunostaining, neurospheres were cultured on poly-D-lysine (15 μg/ml)-coated and fibronectin (1 μg/ml, Invitrogen, Carlsbad, CA, www.invitrogen.com)-coated eight-well chamber slides (Nalge Nunc, Rochester, NY, www.nalgenunc.com) in the presence of basic fibroblast growth factor (bFGF; 10 ng/ml) and epidermal growth factor (EGF; 10 ng/ml) for up to 1 day. Astrocyte differentiation was induced in NSC cultures by changing the medium to Dulbecco's modified Eagle's medium (DMEM; Nacalai Tesque, Kyoto, Japan, www.nacalai.co.jp/global) with 10% fetal calf serum (FCS) for three weeks. OPC differentiation was induced by culturing NSCs in DMEM containing platelet-derived growth factor AA (10 ng/ml), bFGF (2 ng/ml), and 0.25% FCS (OPC medium) and purified by sequential immunopanning, as described previously [17]. The human oligodendroglioma cell line Hs683 and GBM line U251, both of which were purchased from ATCC (Manassas, VA, www.atcc.org), were cultured in DMEM supplemented with 10% FCS, 100 units/ml penicillin G, and 100 μg/ml streptomycin (Nacalai Tesque, Japan).

Transfection

Transfection of NSCs, astrocytes, OPCs, and human GICs (hGICs) was performed using the Nucleofector procedure, according to the supplier's instructions (Lonza, Cologne, Germany, www.lonzabio.com). In brief, 2 × 106 cells were suspended in the mouse NSC nucleofector solution (100 μl) with 10 μg vectors, and then were transfected using the nucleofector device. Transfected cells were cultured in their optimized medium.

Immunostaining

Immunostaining was carried out as described previously [17]. The following antibodies were used to detect antigens: mouse anti-glial fibrillary acidic protein (GFAP; 1:500; Millipore, Billerica, MA, www.millipore.com, 1:400; Sigma for human cells), rat anti-Nestin (1:1,000; BD Pharmingen, Franklin Lakes, NJ, 1:200; Millipore for human cells), mouse anti-MAP2(a+b) (1:200; Abcam, Cambridge, MA, www.abcam. com), mouse anti-beta tubulin isotype III (1:400; Sigma for human cells), mouse A2B5 (1:2; hybridoma supernatant; ATCC), mouse anti-galactocerebroside (GC; 1:2; hybridoma supernatant; ATCC), rat anti–green fluorescence protein (anti-GFP; 1:1,000; Nacalai Tesque), rabbit anti-prominin1 (1:100; Abcam), and rabbit anti-human CD133 (1:50; Abcam). The antibodies were detected with goat anti-rat IgG-A488 (1:400; Invitrogen), goat anti-mouse IgG-Cy3 (1:400; Jackson ImmunoResearch, West Grove, PA, www.jacksonimmuno.com), goat anti-rabbit IgG-Cy3 (1:400; Jackson ImmunoResearch), goat anti-mouse IgG-A488 (1:400; Invitrogen), and goat anti-mouse IgM-Texas Red (1:400; Jackson ImmunoResearch). To visualize nuclei, the cells were counterstained with 4′,6-diamidino-2-phenylindole (1 μg/ml).

Paraffin-embedded tumors were sectioned at 6-μm thickness. Antigens were retrieved by HistoVT One according to the supplier's instructions (Nacalai Tesque). Endogenous peroxidase activity was inactivated by applying 0.3% H2O2 for 15 minutes at room temperature (RT). The sections were then pretreated with blocking solution (2% skim milk, 0.3% Triton X-100, phosphate-buffered saline [PBS]) for 30 minutes at RT, and incubated with mouse anti-epidermal growth factor receptor vIII (EGFRvIII) antibody clone DH8.3 (1:20, Novocastra) for 30 minutes at RT. The antibodies were visualized with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, www.vectorlabs.com) and diaminobenzidine (Vector Laboratories).

Proliferation Assay

Two thousand cells were cultured in 100 μl of culture medium in each well of 96-well plates. To examine cell proliferation, the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed as follows. Ten microliters of MTT (5 mg/ml, Nacalai Tesque) was added to each well on days 0, 2 or 3, and 4 in vitro. The cells were incubated for 4 hours, the medium was replaced with 100 μl of DMSO, the cells were dissociated, and cell proliferation was quantified on a Benchmark microplate reader (Bio-Rad, Hercules, CA, www.bio-rad.com) with the absorption spectrum at 570 nm.

Soft Agar Assay

A soft agar assay was performed to examine whether the transfected cells could proliferate anchorage independently. The transfected cells were suspended in 0.3% top agar made with the optimized medium and layered onto 0.6% bottom agar made with the same medium. After the top agar had polymerized, culture medium was added, and the cells were cultured for 20 days with medium changes every three days.

Reverse Transcription Polymerase Chain Reaction (RT-PCR)

RT-PCR was carried out as described [4]. Dimethylsulfoxide (DMSO) or Betaine was added to the reaction mixture for olig1 (5% DMSO), olig2 (5% DMSO), contactin1 (5% DMSO), sox2 (10% DMSO), nestin (5% DMSO), and egfrvariant III (egfrvIII) (0.5 M Betaine). Cycle parameters were 20 seconds at 94°C, 40 seconds at 58°C, and 45 second at 72°C for 35 cycles. For gapdh, they were 15 seconds at 94°C, 30 seconds at 53°C, and 90 seconds at 72°C for 22 cycles.

The following oligonucleotide DNA primers were synthesized. For exogenous hras, the 5′ primer was 5′-ATGACA GAATACAAGCTTGTGGTG-3′, and the 3′ primer was 5′-ATTAACCCTCACTAAAGGGAAG-3′. For amphiregulin (areg), the 5′ primer was 5′-ATTCAGTCAGAGTTGAA CAGGT-3′, and the 3′ primer was 5′-ACTTTTCTCCA CACCGTTCACC-3′. For nephroblastoma overexpressed gene (nov), the 5′ primer was 5′-GCATTAACTGCATTGAGCA GAC-3′, and the 3′ primer was 5′-TCTTGGAGGAAGGCCT CATTG-3′. For prominin1 (prom1), the 5′ primer was 5′-AGGCTACTTTGAACATTATCTGCA-3′, and the 3′ primer was 5′-GGCTTGTCATAACAGGATTGT-3′. For twist homolog 2 (twist2), the 5′ primer was 5′-ATGGAGGAGGGCTC CAGCTC-3′, and the 3′ primer was 5′-CTAGTGGGAGGCG GACATGG-3′. For Kruppel-like factor 4 (klf4), the 5′ primer was 5′-CACATGAAGCGACTTCCCCC-3′, and the 3′ primer was 5′-TTGATGTCCGCCAGGTTGAAG-3′. For muscleblind-like 3 (mbnl3), the 5′ primer was 5′-CTGTGACAA TCTGCATGGATTAC-3′, and the 3′ primer was 5′-TTG CCTGTAGTTGTTGGAACGTA-3′. For S100 calcium binding protein A6 (s100a6), the 5′ primer was 5′-ATGG CATGCCCTCTGGATCAG-3′, and the 3′ primer was 5′-TTATTTCAGAGCTTCATTGTAGATC-3′. For paired related homeobox 1 (prrx1), the 5′ primer was 5′-CAGGCCTTG GAGCGTGTCTT-3′, and the 3′ primer was 5′-TCAGTT GACTGTTGGCACCTG-3′. For high mobility group AT-hook 2 (hmga2), the 5′ primer was 5′-ATGAGCGCAC GCGGTGAGGG-3′, and the 3′ primer was 5′-CTAATC CTCCTCTGCGGACTC-3′. For prostaglandin-endoperoxide synthase 2 (ptgs2), the 5′ primer was 5′-CATTCTTTGCC CAGCACTTCA-3′, and the 3′ primer was 5′-GTGGCATA CATCATCAGACCA-3′. For contactin1, the 5′ primer was 5′-GTCACCAGCCAGGAGTACTC-3′, and the 3′ primer was 5′-CAGGAGCAAGCTGAGGAGAC-3′. For NK2 homeobox 2 (nkx2.2), the 5′ primer was 5′-ATGTCGCTGACCAACA CAAAGA-3′, and the 3′ primer was 5′-TCACCAAGTC CACTGCTGGGCCTG-3′. For myelin basic protein (mbp), the 5′ primer was 5′-ATGGCATCACAGAAGAGACCCT-3′, and the 3′ primer was 5′-CTGTCTCTTCCTCCCAGCTTAAA-3′. For proteolipid protein (plp), the 5′ primer was 5′-GACAAGTTTGTGGGCATCACC-3′, and the 3′ primer was 5′-TCGGCCCATGAGTTTAAGGAC-3′. For lipoma HMGIC fusion partner-like 3 (lhfpl3), the 5′ primer was 5′-GCTCTTCCACTACTGCATCGG-3′, and the 3′ primer was 5′-ATTCCGATGATAGCCAGGATGT-3′. For breast carcinoma amplified sequence 1 (bcas1), the 5′ primer was 5′-GAAAGACTCCAGCTGCCAAAC-3′, and the 3′ primer was 5′-ATCCGCTTTGGTCCCAGGCC-3′. For G0/G1switch 2 (g0s2), the 5′ primer was 5′-CCTGGCCAAGGAGATGATGGC-3′, and the 3′ primer was 5′-CTAGGAGGCGTGCTGCCGG-3′. For protein phosphatase 2 (formerly 2A), regulatory subunit B, beta (ppp2r2b), the 5′ primer was 5′-TTCAGCCACAGTGGGAGGTA-3′, and the 3′ primer was 5′-TTTGCTAAAGTCCAGACTGTC-3′. For nucleosome assembly protein 1-like 5 (nap1l5), the mouse 5′ primer was 5′-ATGGCCGACCCCGAGAAGCAG-3′, the human 5′ primer was 5′- ATGGCCGACTCGGAAAACCA-3′, and the 3′ primer was 5′-GAGCTCTTGGATCTTGGCGAG-3′. For heparin-binding EGF-like growth factor (hbegf), the mouse 5′ primer was 5′- CTTTCTCCTCCAAGCCACAAG-3′, and the 3′ primer was 5′- CCATGCCCAACTTCACTTTCTC-3′. For epiregulin (ereg), the mouse 5′ primer was 5′- CATCTAC CTGGTGGACATGAG-3′, and the 3′ primer was 5′- CTG AGGTCACTCTCTCATATTC-3′. For egfr, the mouse 5′ primer was 5′- GATGAAAGAATGCATTTGCCAAG-3′, and the 3′ primer was 5′- GGGGCTGATTGTGATAGACAGG-3′. For egfrvIII, the mouse 5′ primer was 5′-ATGCGACCCT CCGGGACGG-3′, and the 3′ primer was 5′-ATTCCGTTA CACACTTTGCGGC-3′. The primers for nestin, musashi1, sox2, olig1, olig2, mash1, and gapdh were described previously [16].

Microarray Hybridization and Data Processing

Five micrograms of total RNA were labeled with biotin by in vitro transcription using the One-Cycle Target Labeling procedure (Affymetrix, Santa Clara, CA, www.affymetrix.com). The cRNA was subsequently fragmented and hybridized to the GeneChip Mouse Genome 430 2.0 array (Affymetrix), according to the manufacturer's instructions. The microarray image data were processed with the GeneChip Scanner 3000 (Affymetrix) to generate cell library (CEL) data. The CEL data were then subjected to analysis with the dChip software [18], which can normalize and process multiple datasets simultaneously. Data were normalized within the respective groups, according to the program's default setting. Statistical significance was determined by the eBayes method [19] and by ANOVA for two- and three-sample comparisons, respectively. The q value, which is an extension of a quantity called the “false discovery rate (FDR),” was examined to evaluate the genome-wide tests of significance [20, 21]. FDR < 0.1.

Human Brain Tumors

Eleven GBMs, five anaplastic astrocytomas, five anaplastic oligodendrogliomas (AOs), three anaplastic oligoastrocytomas, and two hGICs, hGIC1 and hGIC2, were used following the research guidelines of the RIKEN Center for Developmental Biology and Kumamoto University Graduate School of Medical Science. The detailed characterization of the hGICs will be reported elsewhere (T.T and T.K., unpublished data). Poly(A)+ RNA was prepared from glioma spheres using a QuickPrep mRNA Purification Kit (GE Healthcare, Little Chalfont, U.K., www.gehealthcare.com). Human control brain total RNA was purchased from Invitrogen. The cDNAs were synthesized using a Transcription First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland, http://www.roche.com).

Flow Cytometry

Cells transfected with vectors or immunolabeled for Prom1 (1:200; Abcam) were analyzed in a JSAN cell sorter (Bay Bioscience, Kobe, Japan, www.baybio.co.jp) using dual-wavelength analysis (488 nm solid-state laser and 638-nm-semiconductor laser). Propidium iodide-positive (i.e., dead) cells were excluded from the analysis.

Vector Construction

Full-length mouse HRas was amplified from mouse NSC cDNA using RT-PCR and Phusion polymerase (Finnzymes, Espoo, Finland), according to the manufacturer's instructions, and was cloned into a pDrive vector (Qiagen, Venlo, Netherlands, www.qiagen.com). HRasL61 was made by replacing the glycine at codon 61 with leucine, by PCR. The nucleotide sequences were verified using the BigDye Terminator Kit version 3.1 (Applied Biosystems, Foster City, CA, www.appliedbiosystems.com) and ABI sequencer model 3130xl (Applied Biosystems). HRasL61 cDNA was inserted into the pCMS-EGFP vector, resulting in pCMS-EGFP-HRasL61.

The following oligonucleotide DNAs were synthesized. For the full-length mouse HRas, the 5′ primer was 5′-TGAATTCGCCACCATGACAGAATACAAGCTTGTGGTG-3′ and the 3′ primer was 5′-ACTCGAGTCAGGACAGCACACATTTGCAG-3′. For HRasL61, the 5′ primer was 5′-ACAGCAGGTCTAGAAGAGTATA-3′ and the 3′ primer was 5′-TATACTCTTCTAGACCTGCTGT-3′.

Intracranial Cell Transplantation into the Brain of Nude Mice

Control cells and transfected cells were suspended in 5 μl of culture medium and injected into the brain of 5- to 8-week-old female nude mice that had been anesthetized with 10% pentobarbital. The stereotactic coordinates of the injection site were 2-mm forward from lambda, 2-mm lateral from the sagittal suture, and 5-mm deep.

Brain Fixation and Histopathology

The dissected mouse brains were fixed in 4% paraformaldehyde at 4°C overnight. After fixation, the brains were cryoprotected with 12%–18% sucrose in PBS and embedded in Tissue-Tek OCT compound (Miles, Elkhart, IN). Coronal sections (10-μm thick) were prepared from the cerebral cortex and stained with H&E using a standard technique.

Oral Administration of Drugs to Mice That Had Been Injected with Transformed Cells

The EGFR-signaling inhibitor, Gefitinib (200 mg/kg/day; AstraZeneca, London, U.K., www.astrazeneca.com), and Ptgs2 inhibitor, celecoxib (60 mg/kg/day; Astellas, Tokyo, Japan, www.astellas.com), were orally administered every day after the operation, using a disposable flexible-type animal-feeding needle (Fuchigami, Kyoto, Japan).

Statistical Analysis

The survival data were analyzed for significance by Kaplan-Meier methods using GraphPad Prism version 4 software (p values were calculated with the log-rank test, GraphPad Software, La Jolla, CA, www.graphpad.com).

RESULTS

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

The Combination of Ras Activation and p53 Deletion Transforms NSCs and OPCs, but Not astrocytes, into GIC-like Cells

We prepared NSCs, astrocytes, and OPCs as described previously [22–24]. Over 95% of the NSCs, 98% of the astrocytes, and 95% of the OPCs were positive for their respective specific markers, Nestin, GFAP, and A2B5 (Fig. 1A, 1B). We transfected these cell populations with either a control vector or a HRasL61 expression vector [25], and established cell lines (NSC-C, AST-C, OPC-C, NSC-L61, AST-L61, and OPC-L61), which maintained their original marker immunoreactivities, although the transfected astrocytes lost their GFAP immunoreactivity during culture, as described previously [9] (Fig. 1C, 1D). We first examined whether Ras activation could induce the transformation of NSCs, astrocytes, or OPCs. Both NSC-L61 and OPC-L61 formed colonies in soft agar, whereas AST-L61 did not, although Ras activation significantly accelerated the proliferation of all the transfected lines, determined by the MTT assay (Fig. 1E–1J). These data suggest that the combination of Ras activation and p53 deletion transforms NSCs and OPCs but not astrocytes.

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Figure 1. Characterization of HRasL61-transfected p53-deficient NSCs, ASTs, and OPCs. (A):p53-deficient NSCs, ASTs, and OPCs immunolabeled for neural lineage markers (red) and 4′,6-diamidino-2-phenylindole (blue). Scale bar = 15 μm. (B–D): Proportion of neural lineage marker-positive cells in untransfected cells, NSCs, ASTs, and OPCs (B), control vector-transfected cells NSC-C, AST-C, and OPC-C (C), and HRasL61 vector-transfected cells NSC-L61, AST-L61, and OPC-L61 (D), are shown as the mean ± SD of three cultures. (E–G): Proliferation of untransfected (dotted black line) and HRasL61 vector-transfected (red line) cells, determined using the 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results shown are the mean ± SD of three cultures. (H–J): NSC-L61 (H) and OPC-L61 (I), but not AST-L61 (J), formed colonies in soft agar. Abbreviations: AST, astrocyte; GC, galactocerebroside; GFAP, glial fibrillary acidic protein; MAP2, microtubule associated protein 2; NSC, neural stem cell; OPC, oligodendrocyte precursor cell.

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To investigate the tumor-forming ability of the transfected cells, we injected 1,000 cells of each type into the brain of nude mice. Mice that received either NSC-L61 (n = 10 of 10) or OPC-L61 (n = 9 of 9) developed brain tumors and died within 30 days, whereas none that were given injections of 103 AST-L61 (n = 10), or 105 control NSC-C (n = 3), AST-C (n = 3), or OPC-C (n = 3) showed evidence of brain tumorigenesis (Fig. 2A–2C). Although tumors that had characteristics of human astrocytoma, including hypercellularity and pleomorphism, formed in three of eight mice injected with 104 AST-L61 cells (Fig. 3), the secondary transplantation of 104 GFP+ cells did not result in brain tumors, suggesting that AST-L61 does not contain bona fide GIC-like cells or that AST-L61 cells need an additional mutation(s) to transform into GICs.

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Figure 2. NSC-L61 and OPC-L61, but not AST-L61, are enriched in glioma-initiating cell-like cells. (A–C): Survival curves for mice injected with a limiting dilution of NSC-L61 (A), OPC-L61 (B), and AST-L61 (C). (D–O): Brain sections with tumors derived from NSC-L61 (D–G) and OPC-L61s (H–K). Both types of transformed cells invaded the brain parenchyma (green in [D, H]). H&E staining of the tumors showed necrosis (arrowhead in [E, I]), hypercellularity and hypervascularity, multinuclear giant cells (black arrow), and mitotic cells (white arrow) (G, K); these pathological features are similar to human glioblastoma multiforme. H&E staining of secondary tumors (M, O) showed they were phenocopies of the primary tumors (L, N). Scale bar = 100 μm. Abbreviations: AST, astrocyte; NSC, neural stem cell; OPC, oligodendrocyte precursor cell.

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Figure 3. Pathological features of tumors formed by AST-L61. (A): Survival curves for mice injected with control astrocytes (AST-C) or AST-L61. (B): AST-L61 cells invaded the brain parenchyma (green). (C, D): H&E staining of the tumors showed a monotonous fibrous morphology with neither necrosis nor multinucleic giant cells. Scale bar = 100 μm. Abbreviation: AST, astrocyte.

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We further examined the tumorigenic ability of NSC-L61 and OPC-L61. Five of seven mice injected with 100 NSC-L61 cells and three of six mice injected with 10 NSC-L61 cells formed brain tumors (Fig. 2A). In the case of OPC-L61, all of the mice (5/5) injected with 100 cells and three of four mice injected with 10 cells developed brain tumors and died (Fig. 2B). These results suggest that both NSC-L61 and OPC-L61 were highly enriched in GIC-like cells.

NSC-L61 and OPC-L61 Cells Form Transplantable GBM with Features of Human GBM

We next examined the histopathology of the brain tumors formed from NSC-L61 and OPC-L61 (Fig. 2D–2K), to determine their type. The tumors were composed of GFP+ cells, many of which were invading the adjacent brain tissue (Fig. 2D, 2H). H&E staining revealed that the tumors had characteristics of human GBM, including hypercellularity, pleomorphism, multinuclear giant cells, mitosis, and necrosis [26] (Fig. 2E–2G, 2I–2K). To characterize the tumors further, we acutely immunolabeled the dissociated tumor cells for both GFP and neural markers, and found that the tumor-forming cells maintained their original immunoreactivity (Supporting Information Fig. 1).

We then performed serial transplantation experiments using cells from the tumors that had formed in mice injected with either 100 NSC-L61 or 100 OPC-L61 cells. Within four weeks, all of the mice (n = 10 each for NSC-L61- and OPC-L61-derived tumors) that received secondary transplantation developed brain tumors that recapitulated the phenocopy of the primary transplantation (Fig. 2L–2O), indicating that NSC-L61 and OPC-L61 had a high capacity for self-renewal. Moreover, we found that 76% of the NSC-L61 and 44% of the OPC-L61 cells were positive for Prominin1 (Prom1), a putative marker for brain CICs [27, 28] and NSCs [29], whereas only 3% of the control NSCs and 4% of the OPCs were positive for Prom1 (Supporting Information Fig. 2). These data collectively suggest that a significant proportion of both NSC-L61 and OPC-L61 cells are transformed into GIC-like cells.

OPC-L61 Acquired the Expression of NSC-Specific Genes and Extensively Lost the Expression of OPC-Specific Genes

We used gene expression profiling to characterize the changes in gene expression that occurred during the transformation of NSCs and OPCs. We found that the gene expression profile of OPC-L61 was similar to that of control NSC-C and NSC-L61 but different from that of OPC-C (Fig. 4A–4C). A group of genes that were highly expressed in OPC-C showed decreased expression in OPC-L61 and vice versa, suggesting that the OPCs underwent global reprogramming of their gene expression during their transformation to OPC-L61.We then used RT-PCR to assess whether OPC-L61 acquired the expression of NSC-specific genes and lost that of OPC-specific genes (Fig. 4D, 4E). Tissue-specific stem cell markers, including prom1, hmga2, and nov, were expressed in OPC-L61 but not OPC-C, whereas the expression of several OPC-specific genes, contactin1, olig1, olig2, mbp, and plp, significantly decreased in OPC-L61. Moreover, a number of genes, including twist2, klf4, mbnl3, s100a6, prrx1, ptgs2, and ereg, were predominantly expressed in OPC-L61, NSC-C, and NSC-L61, whereas lhfpl3, bcas1, g0s2, ppp2r2b, and nap1l5, all of which were expressed in OPC-C, decreased in OPC-L61. Together, these data suggest that during transformation, the gene expression profile of OPCs changes markedly, and becomes similar to that of NSCs.

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Figure 4. Evidence of global changes in gene expression in OPC transformation. (A): Hierarchical clustering using the Affymetrix murine 430A 2.0 microarray demonstrated that OPC-L61 exhibited a gene expression profile most similar to NSC-L61. (B, C): Diagram of the number of genes that were expressed specifically or commonly in NSC-C, OPC-C, NSC-L61, and OPC-L61. (D, E): Expression analysis of NSC-specific genes (D) and OPC-specific genes (E) in NSC-C, NSC-L61, OPC-C, and OPC-L61 by reverse transcription polymerase chain reaction. gapdh expression was an internal control. Abbreviations: NSC, neural stem cell; OPC, oligodendrocyte precursor cell.

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The Combination of a Ptgs2 Inhibitor and an EGFR-Signaling Inhibitor Prevents the Tumorigenicity of OPC-Derived GIC-Like Cells and Oligodendroglioma-Derived GICs

The finding that OPCs acquired characteristics of NSCs when transformed into GIC-like cells led us to examine whether the upregulated genes in the GIC-like cells include therapeutic targets. Among the candidate genes, we focused on Ptgs2 and factors in the epidermal growth factor (EGF)-signaling pathway, because ptgs2, egfreceptor (egfr), and various types of EGFR ligands, are highly expressed in human gliomas and other cancers and are involved in tumor progression [30, 31]. Indeed, we found that the OPC-L61 and NSC-L61 cells expressed egfr and EGFR ligands areg, hbegf, ptgs2, and ereg (Fig. 5A).

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Figure 5. Combination of a Ptgs2 inhibitor and an epidermal growth factor receptor (EGFR)-signaling inhibitor delays the tumorigenesis of oligodendrocyte lineage-derived GICs. (A–C): Expression analysis of ptgs2, EGFR ligands (areg, hbegf, and ereg), egfr, and egfrvIII in GIC-like cells, hGICs, and human glioma samples (GBM, anaplastic astrocytoma, anaplastic oligodendroglioma, and anaplastic oligoastrocytoma), by reverse transcription polymerase chain reaction. gapdh expression was an internal control. (D–G): Cytotoxic effects of Gefitinib and Celecoxib on induced glioma cells (black column in [D, E]), their parental cells (gray column in [D, E]), hGIC1 (F), and hGIC2 (G). (H–K): Survival curve of NSC-L61- (H), OPC-L61- (I), hGIC1- (J), or hGIC2-injected mice (K), treated with the drug combination (C+G, solid line). Control mice were given water only (dotted line). Abbreviations: GBM, glioblastoma multiforme; GIC, glioma-initiating cell; hGIC, human GIC; NSC, neural stem cell; OPC, oligodendrocyte precursor cell.

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To evaluate the expression of ptgs2, EGFR ligands, and egfr in GICs, we established two hGIC lines, hGIC1 and hGIC2, from primary AO and GBM tissues, respectively, as shown previously [26, 27, 32]. Both of the hGIC lines formed transplantable malignant gliomas in the brains of nude mice when as few as 10 cells were transplanted, suggesting that these lines were also enriched in CICs (T.T. and T.K., unpublished observation). We confirmed that the hGICs expressed ptgs2 and EGFR ligands, whose expression was undetectable in normal brain samples (Fig. 5B). We also found that hGIC1 expressed egfr version III (egfrvIII), a constitutively active form of EGFR that is expressed in malignant glioma, and egfr, whereas hGIC2 expressed egfr only. Moreover, we confirmed the expression of egfrvIII in the human AO and GBM sections and in paraffin-embedded hGIC xenografts by immunohistochemical analysis, as shown previously (Supporting Information Fig. 3) [33]. We further found that ptgs2 and EGFR ligands were predominantly expressed in malignant human gliomas and that 5 of 11 GBM, 2 of 5 AO, and an oligodendroglioma cell line Hs683 expressed egfrvIII (Fig. 5C; Supporting Information Fig. 4). Together, these data suggest that both Ptgs2 and EGFR-signaling pathways are involved in GIC functions.

Inhibitors for Ptgs2 and the EGFR-signaling pathways are used separately as chemotherapeutic reagents for lung cancer, intestinal neoplasm, non-small cell lung carcinoma, and glioma in human. As their use in combination has been shown to block tumorigenesis in both APCMin/+ mice [34] and head-and-neck carcinoma xenografted mice [35], but has not been examined for GBM, we tested the efficacy of their combination in NSC-L61, OPC-L61, hGIC1, and hGIC2. Gefitinib, an EGFR inhibitor, and Celecoxib, a Ptgs2 inhibitor, prevented the proliferation of NSC-L61, OPC-L61, and hGIC cells in a dose-dependent manner, and the combination had additive effects, although control NSCs and OPCs were also affected (Fig. 5D–5G).

We next addressed whether this drug combination blocks the tumorigenesis of NSC-L61, OPC-L61, or hGICs in vivo, as neither Gefitinib nor Celecoxib alone showed any effect on the survival time of NSC-L61- (data not shown), OPC-L61-, or hGIC1-injected mice (Supporting Information Fig. 5). The mean survival time of mice injected with OPC-L61 was significantly longer in the drug-treated group (34.1 ± 9.5 days, p = .005), compared with control (23.0 ± 1.9 days), whereas the combination had little effect on mice injected with NSC-L61 (21.0 ± 2.9 days in drug-treated vs. 19.7 ± 6.9 days in control; Fig. 5H, 5I). In addition, the mean survival time of mice injected with hGIC1 was longer in the drug-treated group (56.2 ± 8.2 days, p = .0027), compared with control mice (44.5 ± 3.4 days), whereas the combination did not increase the mean survival time of mice injected with hGIC2 (37.6 ± 1.1 days in drug-treated vs. 34.6 ± 2.6 days in control; Fig. 5J, 5K). We confirmed that the drug combination could block the tumorigenesis of an oligodendroglioma cell line, Hs683, but not of a GBM line, U251 (Supporting Information Fig. 4). In addition, we found that the knockdown of both egfr and ptgs2 inhibited the proliferation of OPCL61 and hGIC1 cells but not of NSCL61 and hGIC2 cells (data not shown). Together, these results suggest that both EGFR and Ptgs2 are crucial for the proliferation of oligodendrocyte lineage-derived malignant glioma, and that a drug combination targeting both pathways specifically prevents its tumorigenesis.

DISCUSSION

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

Here, we demonstrated that a combination of oncogenic HRasL61 overexpression and p53 deletion, both of whose pathways are frequently mutated in human GBM [8–12], transformed NSCs and OPCs into GIC-like cells. The injection of only 10 of these GIC-like cells formed transplantable GBM with characteristics of human GBM in the brain of nude mice, suggesting that these cells are enriched in CICs and that both NSCs and OPCs are likely cells-of-origin for GBM. In contrast, we found that the same combination could not transform astrocytes; AST-L61 cells did not form either colonies in soft agar or tumors in vivo when 103 of them were transplanted. Interestingly, when 104 AST-L61 cells were transplanted into the brain of nude mice, they formed invasive astrocytoma. Theoretically, because tumors are likely to arise from one or a few CICs, AST-L61 cells are not GIC-like cells but might be premalignant cells that need additional mutation(s) to transform into GIC-like cells, or they might need a specific niche to induce tumorigenesis [36]. It will be interesting to examine which oncogenic mutations can transform astrocytes into GICs.

It has been thought that precursor cells and differentiated cells acquire characteristics of tissue-specific stem cells when they are transformed. Indeed, here we found that when OPCs were transformed into GIC-like cells, they lost their original features and acquired characteristics of NSCs. Although the detailed mechanism of the dedifferentiation caused by transformation remains to be elucidated, it is likely that epigenetic modification plays an important role, as we previously showed that epigenetic modification is involved in OPC reversion [16, 37] and that activation of the Ras-signaling pathway induces alterations in epigenetic modification, chromatin structure, and gene expression [38–42]. As OPCs can be purified and cultured in serum-free medium with defined chemicals and mitogens, OPC transformation might be a good model for investigating the molecular mechanisms of dedifferentiation in tumorigenesis.

Using GIC-like cell models, we demonstrated that oligodendrocyte lineage-derived GBMs (hGIC1) are sensitive to treatment with a Ptgs2 inhibitor and an EGFR-signaling inhibitor in combination, whereas those derived from GBM tissues (hGIC2) were not. As there is much evidence showing that chromosome 1p and 19q loss is a marker for increased sensitivity to standard therapies (chemotherapy and radiation therapy), we examined the 1p and 19q status in the two hGIC lines using the fluorescence in situ hybridization and found that hGIC2 had lost both 1p and 19q, whereas hGIC1 only lost 1p (data not shown), suggesting that the increased sensitivity to the Gefitinib/Celecoxib combination in hGIC1 was not related to the 1p/19q deletions.

There are several possible explanations for why the drug combination appeared more effective in the oligodendrocyte lineage-derived tumors than in GBM tissues. First, EGFR signaling is known to be involved in OPC proliferation and tumorigenesis [43, 44]. Second, the accumulation of Cox2-expressing astrocytes is more often observed in high-grade oligodendroglioma than in GBM [45, 46]. Third, hGIC1 and Hs683 strongly expressed egfrvIII, which was shown by Mellinghoff et al. to cause “pathway addiction” of the tumor cells [47]. Fourth, the National Cancer Institute's Repository for Molecular Brain Neoplasia Data (REMBRANDT) database [48] revealed that the downregulation of either ptgs2 or egfr mRNA is correlated with a significant increase in the survival of oligodendroglioma, but not of GBM patients (Supporting Information Fig. 6). Thus, these data suggest that the combination of these inhibitors with irradiation/chemotherapy may be effective in patients suffering from oligodendrocyte lineage-derived GBMs and point to potential new clinical approaches combining current and GIC-specific therapies.

CONCLUSION

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

In this study, we have shown that induced GBM models are useful for investigating the cells-of-origin of GBM, their characteristics, and therapeutic targets, when combined with studies using hGICs and human glioma samples. Our results also suggest that the therapeutic targets for GBM are different according to the cell-of-origin of the GICs. In future studies, it will be important to find specific markers for classifying GBM according to its cell-of-origin.

Acknowledgements

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

We thank Martin Raff for his critical reading and helpful suggestions on the article, Douglas Sipp and Hazuki Hiraga for their critical reading, Shinichi Aizawa for the p53-deficient mice, and Takeya Kasukawa and Junko Nishio for performing the DNA microarray analysis. This work was supported in part by a Grant-In-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan to T.K.

REFERENCES

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

Supporting Information

  1. Top of page
  2. Abstract
  3. INTRODUCTION
  4. MATERIALS AND METHODS
  5. RESULTS
  6. DISCUSSION
  7. CONCLUSION
  8. Acknowledgements
  9. DISCLOSURE OF POTENTIAL CONFLICTS OF INTEREST
  10. REFERENCES
  11. Supporting Information

Additional supporting information available online.

FilenameFormatSizeDescription
STEM_618_sm_suppinfofigure1.eps3107KSupplemental Figure 1. Immunoreactivity to markers of dissociated tumor-forming cells derived from NSC-L61 and OPC-L61. Tumors were enzymatically dissociated and the dispersed cells were acutely immunolabeled for Nestin, A2B5, GC, GFAP, or MAP2. Each tumor cell type maintained its original immunoreactivity.
STEM_618_sm_suppinfofigure2.eps5615KSupplemental Figure 2. Immunoreactivity of transformed NSCs and OPCs for Prominin1. NSCs, NSC-L61, OPCs, and OPC-L61 were labeled for Prominin1 (Prom1) and analyzed by flow cytometry. The experiments were repeated three times with similar results.
STEM_618_sm_suppinfofigure3.eps6652KSupplemental Figure 3. Expression of EGFRvIII in hGICs. Immunohistochemical analysis for EGFRvIII on paraffin-embedded primary human AO tissue (A) and GBM tissue (B) and on xenografted brain tumors derived from hGIC1 (C) and hGIC2 (D). Scale bar 100 μm.
STEM_618_sm_suppinfofigure4.eps5719KSupplemental Figure 4. Human oligodendroglioma cell line Hs683 expresses EGFRvIII and is sensitive to the combination of Celecoxib and Gefitinib. (A) Expression analysis of egfrvIII in Hs683 and U251. gapdh expression was an internal control. (B-E) H&E staining of the xenografted gliomas derived from U251 (B,D) and Hs683 (C,E) treated with water alone (control) or the combination of Celecoxib and Gefitinib (drugs). Scale bar 100 μm.
STEM_618_sm_suppinfofigure5.eps7653KSupplemental Figure 5. Celecoxib or Gefitinib alone could not extend the survival time of mice injected with either OPCL61 or hGIC1. Survival curve of OPC-L61- (A) or hGIC1-injected mice (B) treated with either Celecoxib (dashed line) or Gefitinib (dotted line). Control mice were given water only (solid line).
STEM_618_sm_suppinfofigure6.eps5631KSupplemental Figure 6. Relationship between the expression of ptgs2 and egfr and the survival of glioma patients. Clinical data from the REMBRANDT database indicates that lower egfr (A) and ptgs2 (B) mRNA levels in oligodendrogliomas are correlated with longer patient survival rates, but not with the survival rates of GBM patients.

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