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Tissue-Specific Stem Cells
Article first published online: 19 APR 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 5, pages 871–882, May 2011
How to Cite
Roobrouck, V. D., Clavel, C., Jacobs, S. A., Ulloa-Montoya, F., Crippa, S., Sohni, A., Roberts, S. J., Luyten, F. P., Van Gool, S. W., Sampaolesi, M., Delforge, M., Luttun, A. and Verfaillie, C. M. (2011), Differentiation Potential of Human Postnatal Mesenchymal Stem Cells, Mesoangioblasts, and Multipotent Adult Progenitor Cells Reflected in Their Transcriptome and Partially Influenced by the Culture Conditions. STEM CELLS, 29: 871–882. doi: 10.1002/stem.633
V.D.R.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; C.C.: conception and design, collection and/or assembly of data, data analysis and interpretation; S.A.J. and S.C.: collection and/or assembly of data, data analysis and interpretation; F.U.-M.: data analysis and interpretation, manuscript writing; A.S., S.J.R., F.P.L., S.W.V.G., M.S., and M.D.: data analysis and interpretation; A.L.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; C.M.V.: conception and design, data analysis and interpretation, manuscript writing, final approval of manuscript.
First published online in STEM CELLS EXPRESS March 23, 2011.
Disclosure of potential conflicts of interest is found at the end of this article.
- Issue published online: 19 APR 2011
- Article first published online: 19 APR 2011
- Accepted manuscript online: 23 MAR 2011 04:21PM EST
- Manuscript Accepted: 4 MAR 2011
- Manuscript Received: 13 OCT 2010
- Athersys Inc.
- European Commission. Grant Number: EC-FP6-STREP-STROKEMAP
- Center of Excellence. Grant Number: EF/05/13
- OT. Grant Number: ETH-C0420-OT/09/053
- GOA. Grant Number: EME-C2161-GOA/11/012
- Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen)
- Flemish Fund for Scientific Research (FWO Vlaanderen)
- Adult stem cells;
- Cell culture;
Several adherent postnatal stem cells have been described with different phenotypic and functional properties. As many of these cells are being considered for clinical therapies, it is of great importance that the identity and potency of these products is validated. We compared the phenotype and functional characteristics of human mesenchymal stem cells (hMSCs), human mesoangioblasts (hMab), and human multipotent adult progenitor cells (hMAPCs) using uniform standardized methods. Human MAPCs could be expanded significantly longer in culture. Differences in cell surface marker expression were found among the three cell populations with CD140b being a distinctive marker among the three cell types. Differentiation capacity towards adipocytes, osteoblasts, chondrocytes, and smooth muscle cells in vitro, using established protocols, was similar among the three cell types. However, only hMab differentiated to skeletal myocytes, while only hMAPCs differentiated to endothelium in vitro and in vivo. A comparative transcriptome analysis confirmed that the three cell populations are distinct and revealed gene signatures that correlated with their specific functional properties. Furthermore, we assessed whether the phenotypic, functional, and transcriptome features were mediated by the culture conditions. Human MSCs and hMab cultured under MAPC conditions became capable of generating endothelial-like cells, whereas hMab lost some of their ability to generate myotubes. By contrast, hMAPCs cultured under MSC conditions lost their endothelial differentiation capacity, whereas this was retained when cultured under Mab conditions, however, myogenic capacity was not gained under Mab conditions. These studies demonstrate that hMSCs, hMab, and hMAPCs have different properties that are partially mediated by the culture conditions. STEM CELLS 2011;29:871–882