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FilenameFormatSizeDescription
STEM_654_sm_suppfigure1.tif154KSupporting Figure 1. MTT cell viability assay. Absorbances at 550nm and 690nm were measured. No differences were observed in the use of any of the reagents at the concentration and times determined in mat and methods (section mitochondria quantification) in comparison with control SD and FD cells.
STEM_654_sm_suppfigure2.tif2689KSupporting Figure 2. Mitochondria staining with MitoTracker Red FM of two different SD clones (A and B) and one FD clone (C). Magnification is specified in the figure (40x or 100x).
STEM_654_sm_suppfigure3.tif429KSupporting Figure 3. A and C. OCR measurements with the SeaHorse System. Left graphic shows proliferating FD and SD cells and right graphic shows differentiating SD and FD cells. B and D. ECAR measurements with the SeaHorse System. Left graphic shows proliferating FD and SD cells while right graphic shows differentiating SD and FD cells.
STEM_654_sm_suppfigure4.tif318KSupporting Figure 4. OCR measurements with the SeaHorse System. (A) Graphic shows proliferating FD and SD cells (in presence or absence of stimuli) in 25mM Glucose. (B) Graphic shows proliferating SD and FD cells(in presence or absence of stimuli) in 25mM Galactose.
STEM_654_sm_suppfigure5.tif128KSupporting Figure 5. Measurements of the membrane potential by flow cytometry with JC-1. Note that the quantity of aggregates (red fluorescence) varies depending on the treatment. Mean fluorescence intensity is shown at the upper right side of the graphic.
STEM_654_sm_suppfigure6.tif4691KSupporting Figure 6. Electron microscopy images for SD and FD clones both unstimulated and subjected to the different treatments (left images: low resolution at 8.000x ; right images: high resolution at 25.000x). Graph represents the quantification of mitochondria counted per cell section (mean).

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