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Tissue-Specific Stem Cells
Article first published online: 29 JUN 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 7, pages 1064–1074, July 2011
How to Cite
San Martin, N., Cervera, A. M., Cordova, C., Covarello, D., McCreath, K. J. and Galvez, B. G. (2011), Mitochondria Determine the Differentiation Potential of Cardiac Mesoangioblasts. STEM CELLS, 29: 1064–1074. doi: 10.1002/stem.654
Author contributions: N.S.M., A.M.C., C.C., and D.C.: collection and assembly of data; data analysis and interpretation; K.J.M. and B.G.G.: conception and design; data analysis and interpretation; manuscript writing; final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS May 4, 2011.
- Issue published online: 29 JUN 2011
- Article first published online: 29 JUN 2011
- Accepted manuscript online: 4 MAY 2011 08:09AM EST
- Manuscript Accepted: 11 APR 2011
- Manuscript Received: 1 DEC 2010
- Heart Repair
- Ministerio de Ciencia e Innovación (MICINN). Grant Numbers: SAF2010-15239, SAF2009-07965
- Pro-CNIC Foundation
Additional supporting information available online.
|STEM_654_sm_suppfigure1.tif||154K||Supporting Figure 1. MTT cell viability assay. Absorbances at 550nm and 690nm were measured. No differences were observed in the use of any of the reagents at the concentration and times determined in mat and methods (section mitochondria quantification) in comparison with control SD and FD cells.|
|STEM_654_sm_suppfigure2.tif||2689K||Supporting Figure 2. Mitochondria staining with MitoTracker Red FM of two different SD clones (A and B) and one FD clone (C). Magnification is specified in the figure (40x or 100x).|
|STEM_654_sm_suppfigure3.tif||429K||Supporting Figure 3. A and C. OCR measurements with the SeaHorse System. Left graphic shows proliferating FD and SD cells and right graphic shows differentiating SD and FD cells. B and D. ECAR measurements with the SeaHorse System. Left graphic shows proliferating FD and SD cells while right graphic shows differentiating SD and FD cells.|
|STEM_654_sm_suppfigure4.tif||318K||Supporting Figure 4. OCR measurements with the SeaHorse System. (A) Graphic shows proliferating FD and SD cells (in presence or absence of stimuli) in 25mM Glucose. (B) Graphic shows proliferating SD and FD cells(in presence or absence of stimuli) in 25mM Galactose.|
|STEM_654_sm_suppfigure5.tif||128K||Supporting Figure 5. Measurements of the membrane potential by flow cytometry with JC-1. Note that the quantity of aggregates (red fluorescence) varies depending on the treatment. Mean fluorescence intensity is shown at the upper right side of the graphic.|
|STEM_654_sm_suppfigure6.tif||4691K||Supporting Figure 6. Electron microscopy images for SD and FD clones both unstimulated and subjected to the different treatments (left images: low resolution at 8.000x ; right images: high resolution at 25.000x). Graph represents the quantification of mitochondria counted per cell section (mean).|
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