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Additional Supporting Information may be found in the online version of this article.

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STEM_668_sm_suppfigure1.tif34893KSupporting Figure 1. Examples of prostate cancer tumors grown from three individual patients using chimeric xenograft method with mouse stroma. (A) Patient 13; Gleason 3+4 specimen grafted in mouse host for 14 weeks. (B) Patient 12; Gleason 3+4 specimen grafted in mouse host for 7 weeks. (C) Patient 11; Gleason 3+3 specimen grafted in mouse host for 5 weeks. Note: Pre-graft tissues are included for each patient (top row). 1. Dual staining for p63 (brown) and cytokeratins (CKs) 8/18 (red) shows areas of benign glands (positive for p63; indicated by *) and malignant (negative for p63; indicated by arrows) regions of pre-graft and chimeric grafted tissues. Scale bars for row 1 and 3 (100μm), row 2 (500μm). 2. Heamatoxylin and eosin (H&E) staining shows malignant cells, exhibiting prominent macronucleoli (indicated by arrows) and invasive features. Scale bar (50μm. 3. Dual staining for AMACR (pink) and CKH (high molecular weight cytokeratins marking benign basal cells in brown). Benign regions detected by low or absent AMACR and detectable CKH (indicated by *). Malignant regions detected by AMACR and loss of CKH (indicated by arrows). Scale bar (100 μm). 4. Immunolocalization of Ki-67 (brown) shows tumour cells in chimeric grafts are actively proliferating. Scale bar (100 μm).
STEM_668_sm_suppfigure2.tif34902KSupporting Figure 2.Examples of prostate cancer cells grown from three individual patients using chimeric xenograft method with mouse stroma. (A) Patient 1; Gleason 4+5 specimen grafted in mouse host for 8 weeks. (B) Patient 9; Gleason 4+3 specimen grafted in mouse host for 14 weeks. (C) Patient 6; Gleason 3+3 specimen grafted in mouse host for 8 weeks. Note: Pre-graft tissues are included for each patient (top row). 1. Dual staining for p63 (brown) and cytokeratins (CKs) 8/18 (red) shows areas of benign glands (positive for p63; indicated by *) and malignant (negative for p63; indicated by arrows) regions of pre-graft and chimeric grafted tissues. Scale bars for row 1 and 3 (100μm), row 2 (500μm). 2. Heamatoxylin and eosin (H&E) staining shows malignant cells, exhibiting prominent macronucleoli (indicated by arrows) and invasive features. Scale bar (50μm). 3. Dual staining for AMACR (pink) and CKH (brown). Benign regions detected by low or absent AMACR and detectable CKH (indicated by *). Malignant regions detected by AMACR and loss of CKH (indicated by arrows). Scale bar (100 μm).4. Immunolocalization of Ki-67 (brown) shows tumour cells in chimeric grafts are actively proliferating. Scale bar (100 μm).
STEM_668_sm_suppfigure3.tif6947KSupporting Figure 3. Dual-label centromeres and telomeres in chimeric xenografts of prostate cancer cells co-grafted with mouse stroma. Photomicrographs of H&E and serial section stained by protein nucleic acid FISH probes to dual-label centromeres (green) and telomeres (red) shows the presence of human cancer cells (green and red, indicated by *) surrounded by stroma from co-grafted and/or host-derived mouse cells (red, indicated by arrows). Scale bar (50 μm). Note: FISH analysis is not provided for isolated prostate cancer cells grown without mouse mesenchyme, since these grafts did not generate tumors.
STEM_668_sm_suppinformation.doc53KSupporting Information

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