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Brief Report: Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 26 JUL 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 8, pages 1304–1309, August 2011
How to Cite
Ko, K., Reinhardt, P., Tapia, N., Schneider, R. K., Araúzo-Bravo, M. J., Han, D. W., Greber, B., Kim, J., Kliesch, S., Zenke, M. and Schöler, H. R. (2011), Brief Report: Evaluating the Potential of Putative Pluripotent Cells Derived from Human Testis. STEM CELLS, 29: 1304–1309. doi: 10.1002/stem.671
Author contributions: K.K.: conception and design, collection and assembly of data, data analysis and interpretation and manuscript writing; P.R., R.K.S., M.J.A.-B., D.W.H., B.G., and J.K.: collection and assembly of data; N.T.: conception and design, data analysis and interpretation, and manuscript writing; S.K.: provision of study material or patient biopsies; M.Z.: data analysis and interpretation; H.R.S.: conception and design, data analysis and interpretation, manuscript writing, and final manuscript approval. K.K., P.R., and N.T. contributed equally to this work.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLS EXPRESS June 7, 2011.
- Issue published online: 26 JUL 2011
- Article first published online: 26 JUL 2011
- Accepted manuscript online: 7 JUN 2011 08:24AM EST
- Manuscript Accepted: 21 MAY 2011
- Manuscript Received: 28 MAR 2011
- Max Planck Society
- German Research Foundation (DFG). Grant Number: SPP 1356
- “Pluripotency and Cellular Reprogramming”. Grant Number: SCHO 340/5-1
- Federal Ministry of Education and Research (BMBF)
- “Disease-specific iPS cells”. Grant Number: FKZ 01GN0811
- Human adult germline stem cells;
- Human testicular biopsies;
- Human testis ESC-like cells
Human adult germline stem cells (haGSCs) were established from human testicular biopsies and were claimed to be pluripotent. Recently, the gene expression profile of haGSCs demonstrated that these cells presented with a fibroblast rather than a pluripotent identity. Nevertheless, haGSCs were reported to generate teratomas. In this report, we address this discrepancy. Instead of using haGSCs, which are no longer available for the stem cell community, we used a human testicular fibroblastic cell (hTFC) line that presents with a gene expression profile highly similar to that of haGSCs. Indeed, as shown by microarray analysis, the similarity between hTFCs and haGSCs is comparable to human embryonic stem cell (hESC) lines derived by different laboratories. We argue that the almost identical gene expression profile of hTFCs and haGSCs should result in a very similar if not identical differentiation potential. Strikingly, hTFCs were not able to generate teratomas after injection into nude mice. Instead, they formed a mesenchymal lesion that morphologically resembled the putative haGSC-derived teratomas reported previously. We conclude that haGSCs, which exhibit a profile similar to that of fibroblasts and could not generate teratomas, are not pluripotent. Future work will have to show if pluripotent cells can be derived from human testicular biopsies. Mouse work and certain testicular germ cell tumors indicate that this will be possible. STEM CELLS 2011;29:1304–1309