Complete Meiosis from Human Induced Pluripotent Stem Cells§

Authors


  • Author contributions: C.E.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, final approval of manuscript; N.M.: conception and design, collection and/or assembly of data, data analysis and interpretation; R.V.: collection and/or assembly of data, data analysis and interpretation, manuscript writing; M.B.: collection and/or assembly of data, data analysis and interpretation; E.G., L.G-Q., F.V., and A.G.: collection and/or assembly of data; A.V.: conception and design, final approval of manuscript, J.C.I.B.: conception and design, final approval of manuscript, financial support.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS June 16, 2011.

Abstract

Gamete failure-derived infertility affects millions of people worldwide; for many patients, gamete donation by unrelated donors is the only available treatment. Embryonic stem cells (ESCs) can differentiate in vitro into germ-like cells, but they are genetically unrelated to the patient. Using an in vitro protocol that aims at recapitulating development, we have achieved, for the first time, complete differentiation of human induced pluripotent stem cells (hiPSCs) to postmeiotic cells. Unlike previous reports using human ESCs, postmeiotic cells arose without the over-expression of germline related transcription factors. Moreover, we consistently obtained haploid cells from hiPSCs of different origin (keratinocytes and cord blood), produced with a different number of transcription factors, and of both genetic sexes, suggesting the independence of our approach from the epigenetic memory of the reprogrammed somatic cells. Our work brings us closer to the production of personalized human gametes in vitro. STEM CELLS 2011;29:1186-1195

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