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Version of Record online: 26 JUL 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 8, pages 1219–1230, August 2011
How to Cite
Natesan, S., Wrice, N. L., Baer, D. G. and Christy, R. J. (2011), Debrided Skin as a Source of Autologous Stem Cells for Wound Repair. STEM CELLS, 29: 1219–1230. doi: 10.1002/stem.677
Disclaimer: The opinions and assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of Defense or Department of Army. The authors are employees of the U.S. government, and this work was prepared as part of their official duties. This research was funded by the U.S. Army Medical Research and Materiel Command.
Author contributions: S.N.: conception and design, collection and assembly of data, manuscript writing, data analysis and interpretation; N.L.W.: collection and assembly of data, data analysis and interpretation; D.G.B.: conception and design, manuscript writing; R.J.C.: conception and design, data analysis and interpretation, manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS June 14, 2011.
- Issue online: 26 JUL 2011
- Version of Record online: 26 JUL 2011
- Accepted manuscript online: 14 JUN 2011 10:28AM EST
- Manuscript Accepted: 24 MAY 2011
- Manuscript Received: 12 JAN 2011
- University of Texas Health Science Center at San Antonio
- NIH. Grant Number: NCI P30 CA054174
- Postdoctoral Fellowship Grant from the Pittsburgh Tissue Engineering Initiative (PTEI)
- U.S. Army Medical Research and Materiel Command
- Debrided skin;
- Adipose stem cells;
- Burn wounds;
- Perivascular niche
Major traumatic injuries to the body, such as large surface area burns, limit the availability of autologous stem cell populations for wound repair. This report demonstrates that even after severe burn trauma to the body, resident stem cells present within the subcutaneous adipose tissue survive and are available for therapeutic uses. Debrided skin from wounded areas contains subcutaneous adipose tissue and can yield approximately 1.5 × 105 to 2.5 × 105 cells per milliliter of tissue. This observation indicates that tissue, which is normally discarded, could be a valuable source of stem cells. Initial immunohistochemistry of the debrided tissue localized platelet-derived growth factor receptor beta+ (PDGFR-β+) cells to perivascular niches of vascular beds. It was immunophenotypically confirmed that the cell isolates are stem cells and designated as debrided skin adipose-derived stem cells (dsASCs). Gene expression analysis of stem cell specific transcripts showed that the dsASCs maintained their stemness over serial passages. Furthermore, dsASCs were able to differentiate into adipogenic, osteogenic, and vascular cell lineages. Finally, an in vivo excision wound model in athymic rats demonstrated that the dsASCs are engrafted within a wound bed after 12 days. These data provide the first evidence that subcutaneous adipose tissue from discarded burned skin contains a viable population of stem cells that can be used for wound repair and skin regenerative therapies. STEM CELLS 2011;29:1219–1230