Novel Stem/Progenitor Cell Population from Murine Tracheal Submucosal Gland Ducts with Multipotent Regenerative Potential§

Authors

  • Ahmed E. Hegab,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Vi Luan Ha,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Jennifer L. Gilbert,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Kelvin Xi Zhang,

    1. Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, USA
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  • Stephen P. Malkoski,

    1. Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado Denver Health Sciences Center, Aurora, Colorado, USA
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  • Andy T. Chon,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Daphne O. Darmawan,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Bharti Bisht,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Aik T. Ooi,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Matteo Pellegrini,

    1. Department of Molecular, Cellular, and Developmental Biology, University of California Los Angeles, Los Angeles, California, USA
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  • Derek W. Nickerson,

    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
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  • Brigitte N. Gomperts

    Corresponding author
    1. Department of Pediatrics, David Geffen School of Medicine at University of California Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, California, USA
    2. Jonsson Comprehensive Cancer Center, Los Angeles, California, USA
    3. Department of Medicine, Division of Pulmonary and Critical Care Medicine, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, USA
    4. Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, California, USA
    • 10833 Le Conte Ave., A2-410 MDCC, Los Angeles, California 90095, USA
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    • Telephone: 1-310-825-6708; Fax: 1-310-206-8089


  • Author contributions: A.E.H.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; V.L.H., J.L.G., A.T.C., and D.O.D.: collection and/or assembly of data; K.X.Z., B.B., A.T.O., and M.P.: data analysis and interpretation; S.P.M.: provision of study material; D.W.N.: collection and/or assembly of data, data analysis and interpretation; B.N.G.: conception and design, collection and/or assembly of data, data analysis and interpretation, manuscript writing, and final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS June 24, 2011.

Abstract

The airway epithelium is in direct contact with the environment and therefore constantly at risk for injury. Basal cells (BCs) have been found to repair the surface epithelium (SE), but the contribution of other stem cell populations to airway epithelial repair has not been identified. We demonstrated that airway submucosal gland (SMG) duct cells, in addition to BCs, survived severe hypoxic-ischemic injury. We developed a method to isolate duct cells from the airway. In vitro and in vivo models were used to compare the self-renewal and differentiation potential of duct cells and BCs. We found that only duct cells were capable of regenerating SMG tubules and ducts, as well as the SE overlying the SMGs. SMG duct cells are therefore a multipotent stem cell for airway epithelial repair This is of importance to the field of lung regeneration as determining the repairing cell populations could lead to the identification of novel therapeutic targets and cell-based therapies for patients with airway diseases. STEM CELLS 2011;29:1283–1293

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