Tissue-Specific Stem Cells
Version of Record online: 16 NOV 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 12, pages 2005–2017, December 2011
How to Cite
Stoll, E. A., Habibi, B. A., Mikheev, A. M., Lasiene, J., Massey, S. C., Swanson, K. R., Rostomily, R. C. and Horner, P. J. (2011), Increased Re-Entry into Cell Cycle Mitigates Age-Related Neurogenic Decline in the Murine Subventricular Zone. STEM CELLS, 29: 2005–2017. doi: 10.1002/stem.747
Author contributions: E.A.S.: conception and design, collection and/or assembly of data, data analysis and interpretation, development of mathematical model, and manuscript writing; B.A.H.: collection and/or assembly of data and data analysis and interpretation; A.M.M.: conception and design; J.L.: collection and/or assembly of data; S.C.M. and K.R.S.: development of mathematical model; R.C.R. and P.J.H.: conception and design, financial support, data analysis and interpretation, and manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS September 10, 2011.
- Issue online: 16 NOV 2011
- Version of Record online: 16 NOV 2011
- Accepted manuscript online: 21 SEP 2011 01:14PM EST
- Manuscript Accepted: 10 SEP 2011
- Manuscript Received: 19 JAN 2011
- University of Washington Training
- Developmental Biology. Grant Number: HDO7183-28
- University of Washington Retirement Association Fellowship
- American Foundation for Aging Research
- Mary Gates Undergraduate Fellowship. Grant Numbers: AG029406, NSO46724
Additional Supporting Information may be found in the online version of this article.
|STEM_747_sm_supplFig1.pdf||42K||Supp Figure 1. BrdU+ label-retaining cells are observed in the adult and aged SVZ. Representative images from the subventricular zones of 3mo (A) and 20mo (B) mice injected with 50mg/kg BrdU intraperitoneally for 12 days, then sacrificed after a latency of 28 days. BrdU is labeled in green, SOX2 in red, GFAP in blue; the line indicates 20μm. 45% fewer BrdU+ labelretaining cells are observed in the aged SVZ, compared with young SVZ (C, p<0.05). Fewer BrdU-retaining aged cells colabel with NPC markers SOX2 (D, p<0.01); no significant change was observed in BrdU+GFAP+ cells (D, p>0.05).|
|STEM_747_sm_supplFig2.tif||750K||Supp Figure 2. Number of model-predicted and actual BrdU+ cells over 12 days in vivo.|
|STEM_747_sm_supplFig3.tif||2082K||Supp Figure 3. Schematic describing age-related neurogenic decline. The aged SVZ contains a smaller population of stem-like cells, which give rise to progenitor cells. Aged progenitors undergo more rounds of cell division over time than young progenitors, expanding this population, which is pared down again by decreased survival and neuronal differentiation (shown with X's).|
|STEM_747_sm_suppltable1.tif||599K||Supp Table 1. Summary of data incorporated into in vitro mathematical model.|
|STEM_747_sm_suppltable2.tif||863K||Supp Table 2. Summary of data incorporated into in vivo mathematical model.|
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