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Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics
Article first published online: 16 NOV 2011
Copyright © 2011 AlphaMed Press
Volume 29, Issue 12, pages 1995–2004, December 2011
How to Cite
Liang, Y.-J., Yang, B.-C., Chen, J.-M., Lin, Y.-H., Huang, C.-L., Cheng, Y.-Y., Hsu, C.-Y., Khoo, K.-H., Shen, C.-N. and Yu, J. (2011), Changes in Glycosphingolipid Composition During Differentiation of Human Embryonic Stem Cells to Ectodermal or Endodermal Lineages. STEM CELLS, 29: 1995–2004. doi: 10.1002/stem.750
Author contributions: Y.-J.L.: conception and design, collection of data, data analysis and interpretation, and manuscript writing; B.-C.Y., J.-M.C., Y.-H.L., C.-L.H., Y.-Y.C., and C.-Y.H.: collection of data and data analysis and interpretation; K-H.K. and C-N.S.: conception and design and provision of study material; J.Y.: conception and design, data analysis and interpretation, manuscript writing, and final approval of manuscript.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS September 28, 2011.
- Issue published online: 16 NOV 2011
- Article first published online: 16 NOV 2011
- Accepted manuscript online: 28 SEP 2011 02:53PM EST
- Manuscript Accepted: 17 SEP 2011
- Manuscript Received: 11 JUL 2011
- Genomics Research Center
- Academia Sinica and the National Science Council, Taiwan. Grant Number: NSC99-3111-B-001-006
- NSC. Grant Number: NSC99-3111-B-001-003
- NSC. Grant Number: NSC 98-3112-B-001-025
- Human embryonic stem cells;
- Matrix-assisted laser desorption ionization mass spectrometry;
- Surface markers
Glycosphingolipids (GSLs) are ubiquitous components of cell membranes that can act as mediators of cell adhesion and signal transduction and can possibly be used as cell type-specific markers. Our previous study indicated that there was a striking switch in the core structures of GSLs during differentiation of human embryonic stem cells (hESCs) into embryoid body (EB), suggesting a close association of GSLs with cell differentiation. In this study, to further clarify if alterations in GSL patterns are correlated with lineage-specific differentiation of hESCs, we analyzed changes in GSLs as hESCs were differentiated into neural progenitors or endodermal cells by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) and tandem mass spectrometry (MS/MS) analyses. During hESC differentiation into neural progenitor cells, we found that the core structures of GSLs switched from globo- and lacto- to mostly ganglio-series dominated by GD3. On the other hand, when hESCs were differentiated into endodermal cells, patterns of GSLs totally differed from those observed in EB outgrowth and neural progenitors. The most prominent GSL identified by the MALDI-MS and MS/MS analysis was Gb4Ceramide, with no appreciable amount of stage-specific embryonic antigens 3 or 4, or GD3, in endodermal cells. These changes in GSL profiling were accompanied by alterations in the biosynthetic pathways of expressions of key glycosyltransferases. Our findings suggest that changes in GSLs are closely associated with lineage specificity and differentiation of hESCs. STEM CELLS 2011;29:1995–2004.