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Additional Supporting Information may be found in the online version of this article.

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STEM_759_sm_suppinfofig1.tif8291KSupplemental Figure S1. Isolation of myogenic progenitor cells using FACS. (A) Plots showing gating used for MPC isolation by flow cytometry. MPCs were isolated by gating for the nucleated, live cell fraction (Hoechst+ and PI-, respectively), followed by exclusion of hematopoietic and endothelial cells (assessed by CD45- and CD31-, respectively), leaving MPCs exclusively within the Sca1- Alpha7 integrin+ population.
STEM_759_sm_suppinfofig2.tif2050KSupplemental Figure S2. Quantification of absolute values of sorted WT and Cd34?/? MPCs in multiple experiments. (A) LacZ+ MPCs were sorted and used as internal controls injected with WT/GFP+ or Cd34−/−/GFP+ in three independent experiments. Total numbers of LacZ+ fibers were determined. Error bars represent ± SEM for n = 3-5 mice per experiment. (B) Total numbers of GFP+ fibers from WT or Cd34−/− transplanted MPCs were determined in four independent experiments. Error bars represent ± SEM for n = 3-5 mice per experiment. (C) Frequency of Myf5/LacZ+ cells in WT and Cd34−/& minus; sorted MPCs in four independent experiments. Error bars represent ± SEM for n = 3 mice per experiment.
STEM_759_sm_suppinfofig3.tif6527KSupplemental Figure S3. Cd34−/− satellite cells efficiently cross the basal lamina on cultured single fibers. (A) WT and Cd34−/− single fibers were cultured and harvested at 0, 24, 48, and 72 hours post-culture. Immunofluorescent staining for satellite cells (Pax7) and the basal lamina (laminin) was performed and the location of satellite cells relative to the basal lamina was determined (Pax7, red; laminin, green). Representative images are shown. Bar graphs on the right show the relative proportion of satellite cells below or above the basal lamina in WT and Cd34−/− groups, n = 3 animals. Scale bar = 25 μm.
STEM_759_sm_suppinfovideo1.mpg1799KSupplemental online video S1. Live videomicroscopy imaging of WT satellite cell motility. Live imaging of satellite cells from isolated WT fibers was performed during the 24-48 hours period in culture.
STEM_759_sm_suppinfovideo2.mpg1564KSupplemental online video S2. Live videomicroscopy imaging of Cd34−/− satellite cell motility. Live imaging of satellite cells from isolated Cd34−/− fibers was performed during the 24-48 hours period in culture. Representative videos are shown.

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