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Additional Supporting Information may be found in the online version of this article.

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STEM_773_sm_supplFigure1.tif2919KSupplemental Figure 1. Satellite cell-specific knockout of RBP-J reduces transcript levels of RBP-J and Notch target genes. Quantitative RT-PCR analysis of RBP-J and Notch target transcript levels in SCs from control and RBP-Jcko animals 10 days after tamoxifen treatment. (* p<0.05, ** p<0.01)
STEM_773_sm_supplFigure2.tif2131KSupplemental Figure 2. Histological analysis of muscles from control and RBP-Jcko animals 9 weeks after tamoxifen treatment. Cryosections of TA muscles from control and RBP-Jcko animals 9 weeks after tamoxifen treatment were stained with H&E and displayed no obvious histological differences.
STEM_773_sm_supplFigure3a.tif766KSupplemental Figure 3. Loss of RBP-J does not lead to satellite cell apoptosis. A. Low power (20x) magnification of cryosections of muscles from control and RBP-Jcko mice stained for activated Caspase-3 14 days following tamoxifen treatment. Pax7 staining shows the distribution of SCs. Deletion of RBP-J did not lead to any general increase in the frequency of Caspase-3+ve cells in the muscle. B. Of all the cells that stained positively for activated Caspase-3 in muscles from control and RBP-Jcko mice treated as in panel A, none were either Pax7+ve or in the SC position. This panel shows a higher power (63x) view of a Caspase-3+ve cell (arrow) outside the myofiber's basal lamina.
STEM_773_sm_supplFigure3b.tif344KSupplemental Figure 3. Loss of RBP-J does not lead to satellite cell apoptosis. A. Low power (20x) magnification of cryosections of muscles from control and RBP-Jcko mice stained for activated Caspase-3 14 days following tamoxifen treatment. Pax7 staining shows the distribution of SCs. Deletion of RBP-J did not lead to any general increase in the frequency of Caspase-3+ve cells in the muscle. B. Of all the cells that stained positively for activated Caspase-3 in muscles from control and RBP-Jcko mice treated as in panel A, none were either Pax7+ve or in the SC position. This panel shows a higher power (63x) view of a Caspase-3+ve cell (arrow) outside the myofiber's basal lamina.

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