Additional Supporting Information may be found in the online version of this article.

STEM_777_sm_supplMethods.pdf23KSupplementary Data
STEM_777_sm_supplTables.pdf27KSupplementary Tables
STEM_777_sm_supplFig1.tif1207KFigure S1. Phenotypes apparent in aged Barx2 null mice on the outcrossed and inbred (C57Bl/6) backgrounds. By 15-18 months of age, Barx2−/− mice display hunched backs, splayed-limb stance, disheveled coats and mild alopecia. The phenotypes are typically worse on the C57Bl/6 background (bottom panel). Wildtype and heterozygous mice do not show these signs of musculoskeletal aging until at least 24 months.
STEM_777_sm_supplFig2.tif1020KFigure S2. A. Expression of Pax7 is reduced in muscles from 4-week old Barx2−/− mice relative to Barx2+/− littermates as indicated by semi-quantitative RT-PCR; n – number of mice of each genotype. The housekeeping gene GAPDH is used as a reference standard. B. Barx2−/− myoblast cultures contain fewer cells that strongly express Pax7 than Barx2+/− cultures as indicated by immunostaining; n – number of fields examined. Replicate experiments with independent myoblast isolates gave similar results.
STEM_777_sm_supplMovie1.mp45182KMovie S1. 3D reconstruction of E12.5 muscle section stained with the MF20 antibody (green) and antibodies to Barx2 (red), showing Barx2 in nuclei both within and between fibers. Blue - DAPI.
STEM_777_sm_supplMovie2.mp415272KMovie S2. Typical ambulation of an aged Barx2+/+ mouse.
STEM_777_sm_supplMovie3.mp47845KMovie S3. Typical waddling-type ambulation of an aged Barx2−/− mouse (age-matched with the mouse in Movie S2).

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