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Embryonic Stem Cells/Induced Pluripotent Stem Cells
Article first published online: 18 JAN 2012
Copyright © 2011 AlphaMed Press
Volume 30, Issue 2, pages 140–149, February 2012
How to Cite
Chae, H.-D., Lee, M.-R. and Broxmeyer, H. E. (2012), 5-Aminoimidazole-4-carboxyamide Ribonucleoside Induces G1/S Arrest and Nanog Downregulation via p53 and Enhances Erythroid Differentiation. STEM CELLS, 30: 140–149. doi: 10.1002/stem.778
Author contributions: H.-D.C.: conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing; M.R.L.: collection of data.; H.E.B.: financial support, data analysis and interpretation, and manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 10, 2011.
- Issue published online: 18 JAN 2012
- Article first published online: 18 JAN 2012
- Accepted manuscript online: 10 NOV 2011 04:53PM EST
- Manuscript Accepted: 20 OCT 2011
- Manuscript Received: 18 APR 2011
Additional Supporting Information may be found in the online version of this article.
|STEM_778_sm_supplFigure1.tif||222K||Figure S1. Dose-dependent effect of AICAR on p53, Nanog and SSEA-1 expression in mES cells. (A) R1 mESCs were treated with AICAR at indicated concentrations for 9h. Cell lysates were analyzed for p53 and Nanog expression by immunoblotting. These blots are representatives of three independent experiments. (B) R1 mES cells were treated with AICAR for 2d and analyzed for expression of SSEA-1 by flow cytometry. Values represent mean ± SD (n=3).|
|STEM_778_sm_supplFigure2.pdf||626K||Figure S2. AICAR enhances hemoglobinization of EBs. (A) mESCs were treated with AICAR (0.5 mM) for indicated hours, and then allowed to differentiate to form EBs in the absence of LIF. EBs derived from AICAR-treated mESCs show enhanced hemoglobization on day 8 compared to those from non-treated mESCs. (B) Morphology of EBs formed by differentiation culture for 8d with or without AICAR.|
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