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Article first published online: 18 JAN 2012
Copyright © 2011 AlphaMed Press
Volume 30, Issue 2, pages 187–196, February 2012
How to Cite
Grcevic, D., Pejda, S., Matthews, B. G., Repic, D., Wang, L., Li, H., Kronenberg, M. S., Jiang, X., Maye, P., Adams, D. J., Rowe, D. W., Aguila, H. L. and Kalajzic, I. (2012), In Vivo Fate Mapping Identifies Mesenchymal Progenitor Cells. STEM CELLS, 30: 187–196. doi: 10.1002/stem.780
Author contributions: D.G.: conception and design, collection and assembly of data, manuscript writing, and final approval of manuscript; S.P. collection and assembly of data, data analysis and interpretation, and manuscript writing; B.G.M., D.R., L.W., H.L., and D.J.A.: collection and assembly of data and final approval of manuscript; M.S.K.: collection and assembly of data, final approval of manuscript, and manuscript writing; X.J.: collection and assembly of data and data analysis and interpretation; P.M.: data analysis and interpretation and manuscript writing; D.W.R.: provision of study materials, financial support, and manuscript writing; H.L.A.: data analysis and interpretation, financial support, manuscript writing, and final approval of manuscript.; I.K.: conception and design, collection and assembly of data, data analysis and interpretation, financial support, manuscript writing, and final approval of manuscript. D.G. and S.P. contributed equally to this article.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 14, 2011.
- Issue published online: 18 JAN 2012
- Article first published online: 18 JAN 2012
- Accepted manuscript online: 14 NOV 2011 03:09PM EST
- Manuscript Accepted: 21 OCT 2011
- Manuscript Received: 13 MAY 2011
- NIH/NIAMS. Grant Numbers: AR059315-01, AR055607-01
- NIH/NHLBI. Grant Number: 1RC1HL100569-01
- NIH/NIAMS. Grant Number: AR043457
- Croatian Science Foundation
- Adult stem cells;
- In vivo tracking;
- Marrow stromal cells;
- Mesenchymal stem cells
Adult mesenchymal progenitor cells have enormous potential for use in regenerative medicine. However, the true identity of the progenitors in vivo and their progeny has not been precisely defined. We hypothesize that cells expressing a smooth muscle α-actin promoter (αSMA)-directed Cre transgene represent mesenchymal progenitors of adult bone tissue. By combining complementary colors in combination with transgenes activating at mature stages of the lineage, we characterized the phenotype and confirmed the ability of isolated αSMA+ cells to progress from a progenitor to fully mature state. In vivo lineage tracing experiments using a new bone formation model confirmed the osteogenic phenotype of αSMA+ cells. In vitro analysis of the in vivo-labeled SMA9+ cells supported their differentiation potential into mesenchymal lineages. Using a fracture-healing model, αSMA9+ cells served as a pool of fibrocartilage and skeletal progenitors. Confirmation of the transition of αSMA9+ progenitor cells to mature osteoblasts during fracture healing was assessed by activation of bone-specific Col2.3emd transgene. Our findings provide a novel in vivo identification of defined population of mesenchymal progenitor cells with active role in bone remodeling and regeneration. STEM CELLS 2012; 30:187–196.