SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
STEM_781_sm_supplFigure1.tif377KFigure S1. CFU-FV expanded in vitro do not induce leukemia when transplanted into Stk−/− mice. CFU-FV cells were propagated as described in Figure 2. 5x105 CFUFV were transplanted into Stk−/− mice and 9 week post transplant spleen weight (left) and WBC counts (right) were measured.
STEM_781_sm_supplFigure2.pdf1337KFigure S2. Friend virus LSCs are highly enriched in slow dividing PKH26 hi cells that express CD133. (A) LSCs expanded in HH media were stained with PKH26 and grown for 1 week. The cells were sorted for PKH26 fluorescence and plated in methylcellulose media for LSC colonies or BFU-E colonies. (B) mRNA expression of genes associated with stem cell self renewal was analyzed by RT-PCR analysis of RNA isolated from PKH26hi and PKH26lo LSC fractions sorted by FACS (right). (Left) PKH26hi and PKH26lo LSC populations were sorted by FACS, fixed and stained with anti-Bmi1 and Numb antibodies. Expression of Bmi1 and Numb was analyzed by flow cytometry. (C) LSCs expanded in HH media were stained with anti-CD133 antibody and sorted into CD133+ and CD133- populations by FACS. The two populations were plated in methylcellulose media for LSC colonies or Epoind BFU-E.
STEM_781_sm_suppl_material.pdf22KSupplementary Data

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.