Cancer Stem Cells
Article first published online: 18 JAN 2012
Copyright © 2011 AlphaMed Press
Volume 30, Issue 2, pages 121–130, February 2012
How to Cite
Hegde, S., Hankey, P. and Paulson, R. F. (2012), Self-Renewal of Leukemia Stem Cells in Friend Virus-Induced Erythroleukemia Requires Proviral Insertional Activation of Spi1 and Hedgehog Signaling but Not Mutation of p53. STEM CELLS, 30: 121–130. doi: 10.1002/stem.781
Author contributions: S.H.: conception and design of experiments, data collection and analysis, and manuscript writing; P.H.: conception and design of experiments and manuscript writing; R.F.P.: conception and design of experiments, financial support, data analysis, and manuscript writing.
Disclosure of potential conflicts of interest is found at the end of this article.
First published online in STEM CELLSEXPRESS November 14, 2011.
- Issue published online: 18 JAN 2012
- Article first published online: 18 JAN 2012
- Accepted manuscript online: 14 NOV 2011 03:11PM EST
- Manuscript Accepted: 25 OCT 2011
- Manuscript Received: 20 FEB 2011
- NIH. Grant Number: RO1 DK080040
Additional Supporting Information may be found in the online version of this article.
|STEM_781_sm_supplFigure1.tif||377K||Figure S1. CFU-FV expanded in vitro do not induce leukemia when transplanted into Stk−/− mice. CFU-FV cells were propagated as described in Figure 2. 5x105 CFUFV were transplanted into Stk−/− mice and 9 week post transplant spleen weight (left) and WBC counts (right) were measured.|
|STEM_781_sm_supplFigure2.pdf||1337K||Figure S2. Friend virus LSCs are highly enriched in slow dividing PKH26 hi cells that express CD133. (A) LSCs expanded in HH media were stained with PKH26 and grown for 1 week. The cells were sorted for PKH26 fluorescence and plated in methylcellulose media for LSC colonies or BFU-E colonies. (B) mRNA expression of genes associated with stem cell self renewal was analyzed by RT-PCR analysis of RNA isolated from PKH26hi and PKH26lo LSC fractions sorted by FACS (right). (Left) PKH26hi and PKH26lo LSC populations were sorted by FACS, fixed and stained with anti-Bmi1 and Numb antibodies. Expression of Bmi1 and Numb was analyzed by flow cytometry. (C) LSCs expanded in HH media were stained with anti-CD133 antibody and sorted into CD133+ and CD133- populations by FACS. The two populations were plated in methylcellulose media for LSC colonies or Epoind BFU-E.|
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