Bone Marrow-Derived Mesenchymal Stromal Cells are Attracted by Multiple Myeloma Cell-Produced Chemokine CCL25 and Favor Myeloma Cell Growth in Vitro and In Vivo§

Authors

  • Song Xu,

    1. Stem Cell Laboratory, Division of Clinical Hematology, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium
    2. Department of Hematology and Immunology, Myeloma Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium
    3. Department of Lung Cancer Surgery, Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China
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  • Eline Menu,

    1. Department of Hematology and Immunology, Myeloma Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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  • Ann De Becker,

    1. Stem Cell Laboratory, Division of Clinical Hematology, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium
    2. Department of Hematology and Immunology, Myeloma Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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  • Ben Van Camp,

    1. Department of Hematology and Immunology, Myeloma Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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  • Karin Vanderkerken,

    1. Department of Hematology and Immunology, Myeloma Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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  • Ivan Van Riet

    Corresponding author
    1. Stem Cell Laboratory, Division of Clinical Hematology, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium
    2. Department of Hematology and Immunology, Myeloma Center, Vrije Universiteit Brussel (VUB), Brussels, Belgium
    • Stem Cell Laboratory, Division of Clinical Hematology, UZ Brussel, Laarbeeklaan 101, B-1090 Brussels, Belgium
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    • Telephone: +32-02-477-6701; Fax: +32-02-477-6728


  • Author contributions: S.X.: conception and design, assembly of data, data analysis and interpretation, and manuscript writing, E.M.: provision of study material, data analysis and interpretation, and manuscript writing, A.D.B.: provision of study material and data analysis and interpretation, B.V.C.: financial support and final approval of manuscript, K.V.: conception and design, provision of study material, and final approval of manuscript; I.V.R.: conception and design, financial support, and final approval of manuscript.

  • Disclosure of potential conflicts of interest is found at the end of this article.

  • §

    First published online in STEM CELLSEXPRESS November 18, 2011.

Abstract

Multiple myeloma (MM) is a malignancy of terminally differentiated plasma cells that are predominantly localized in the bone marrow (BM). Mesenchymal stromal cells (MSCs) give rise to most BM stromal cells that interact with MM cells. However, the direct involvement of MSCs in the pathophysiology of MM has not been well addressed. In this study, in vitro and in vivo migration assays revealed that MSCs have tropism toward MM cells, and CCL25 was identified as a major MM cell-produced chemoattractant for MSCs. By coculture experiments, we found that MSCs favor the proliferation of stroma-dependent MM cells through soluble factors and cell to cell contact, which was confirmed by intrafemoral coengraftment experiments. We also demonstrated that MSCs protected MM cells against spontaneous and Bortezomib-induced apoptosis. The tumor-promoting effect of MSCs correlated with their capacity to enhance AKT and ERK activities in MM cells, accompanied with increased expression of CyclinD2, CDK4, and Bcl-XL and decreased cleaved caspase-3 and poly(ADP-ribose) polymerase expression. In turn, MM cells upregulated interleukin-6 (IL-6), IL-10, insulin growth factor-1, vascular endothelial growth factor, and dickkopf homolog 1 expression in MSCs. Finally, infusion of in vitro-expanded murine MSCs in 5T33MM mice resulted in a significantly shorter survival. MSC infusion is a promising way to support hematopoietic recovery and to control graft versus host disease in patients after allogeneic hematopoietic stem cell transplantation. However, our data suggest that MSC-based cytotherapy has a potential risk for MM disease progression or relapse and should be considered with caution in MM patients. STEM CELLS 2012; 30:266–279.

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