This article was prepared by a group consisting of both a United States government employee and non-United States government employees, and as such is subject to 17 U.S.C. Sec. 105.
Ethological resolution of behavioural topography and D1-like versus D2-like agonist responses in congenic D5 dopamine receptor mutants: Identification of D5:D2-like interactions†
Article first published online: 24 JAN 2005
Published 2005 Wiley-Liss, Inc.
Volume 55, Issue 4, pages 201–211, 15 March 2005
How to Cite
O'Sullivan, G. J., Kinsella, A., Sibley, D. R., Tighe, O., Croke, D. T. and Waddington, J. L. (2005), Ethological resolution of behavioural topography and D1-like versus D2-like agonist responses in congenic D5 dopamine receptor mutants: Identification of D5:D2-like interactions. Synapse, 55: 201–211. doi: 10.1002/syn.20107
- Issue published online: 24 JAN 2005
- Article first published online: 24 JAN 2005
- Manuscript Accepted: 16 NOV 2004
- Manuscript Received: 29 JUL 2004
- Science Foundation Ireland
- Programme for Research in Third Level Institutions
- Stanley Medical Research Institute
- National Institute of Mental Health. Grant Number: N01MH30003
- dopamine receptor subtypes;
- D5 knockout mice;
- grooming behaviour;
The phenotypic ethogram of congenic dopamine D5 receptor “knockout” mice was evaluated. Each individual topography of behaviour within the natural repertoire was assessed over the extended course of initial exploration of and subsequent habituation to the environment, and following challenge with a series of D1-like agonists. Over initial exploration, D5-null mice evidenced a modest reduction in locomotion and a modest increase in sifting. Subsequent habituation revealed additional phenotypic effects, primarily overall reduction in grooming and delayed habituation of rearing. Among D1-like agonists, A 68930 stimulates both adenylyl cyclase and a putative D1-like receptor coupled to stimulation of phospholipase C-mediated phosphoinositide hydrolysis; conversely, SK&F 83959 stimulates phosphoinositide hydrolysis but not adenylyl cyclase while SK&F 83822 stimulates adenylyl cyclase but not phosphoinositide hydrolysis. Though programmed grooming syntax and episodic seizure activity induced by A 68930 and SK&F 83822 were unaltered, grooming induced by SK&F 83959 was reduced in D5 mutants. Stereotyped, ponderous locomotion induced by the D2-like agonist RU 24213 was enhanced in D5 mutants. Phenotypic and pharmacological characterisation of congenic D5-null mice at an ethological level identifies novel functional roles for the D5 receptor in mediating discrete topographies of behaviour relating to exploration, sequential motor coordination, and how these processes change over the course of interaction with and habituation to the environment. Additionally, they indicate the involvement of phosphoinositide hydrolysis and D5:D2-like interactions in regulating these processes. Synapse 55:201–211, 2005. Published 2005 Wiley-Liss, Inc.