Dietary restriction (DR) is one of the promising environmental interventions known to attenuate aging and decrease risk of age-related neurodegenerative disorders. The aim of this study was to assess the effects of DR on expression of α-synuclein, a presynaptic protein involved in pathogenesis of Parkinson's and some other neurodegenerative diseases, in the cortex and hippocampus of adult, middle-aged, late middle-aged, and aged rats. Using Real Time RT-PCR, the authors report that aging regulates the expression of α-synuclein in a tissue-specific manner and that long-term DR reverts the late age-related changes of α-synuclein expression. Synapse 61:790–794, 2007. © 2007 Wiley-Liss, Inc.