Increased expression of the NR2A NMDA receptor subunit in the prefrontal cortex of rats reared in isolation
Article first published online: 16 JUN 2009
Copyright © 2009 Wiley-Liss, Inc.
Volume 63, Issue 10, pages 836–846, October 2009
How to Cite
Turnock-Jones, J. J., Jennings, C. A., Robbins, M. J., Cluderay, J. E., Cilia, J., Reid, J. L., Taylor, A., Jones, D. N.C., Emson, P. C. and Southam, E. (2009), Increased expression of the NR2A NMDA receptor subunit in the prefrontal cortex of rats reared in isolation. Synapse, 63: 836–846. doi: 10.1002/syn.20665
- Issue published online: 16 JUN 2009
- Article first published online: 16 JUN 2009
- Manuscript Accepted: 23 DEC 2008
- Manuscript Received: 24 SEP 2008
- MRC Industrial Collaborative Studentship with GlaxoSmithKline
- animal model;
- prepulse inhibition;
A hypofunction of the N-methyl-D-aspartate (NMDA) receptor has been implicated in the pathophysiology of schizophrenia. Compelling evidence of altered NMDA receptor subunit expression in the schizophrenic brain has not, however, so far emerged. Rats reared in isolation exhibit several characteristics, including disturbed sensory gating, which resemble those seen in schizophrenia. To explore the possibility that NMDA receptor dysfunction may contribute to the behavioral and neurochemical consequences of rearing rats in isolation, we compared NMDA receptor subunit expression in brains of rats which were housed in isolation and which displayed a deficit in prepulse inhibition of the acoustic startle response with that of socially housed controls. An initial microarray analysis revealed a 1.26-fold increase in NR2A transcript in the prefrontal cortex, but not in the nucleus accumbens, of rats reared in isolation compared with those housed socially. In contrast, NR1, NR2B, NR2C, NR2D, NR3A, and NR3B subunit expression was unchanged in either brain area. In a second cohort of animals, in situ hybridization revealed increased NR2A mRNA expression in the medial prefrontal cortex, an observation that was substantiated by increased [3H]CGP39653 binding suggesting that NR2A receptor subunit protein expression was also elevated in the medial prefrontal cortex of the same animals. No changes in expression of NR1 or NR2B subunits were observed at both mRNA and protein level. Altered NR2A subunit expression in the medial prefrontal cortex of rats reared in isolation suggests that NMDA receptor dysfunction may contribute to the underlying pathophysiology of this preclinical model of aspects of schizophrenia. Synapse 63:836–846, 2009. © 2009 Wiley-Liss, Inc.