Neurites from PC12 cells are connected to each other by synapse-like structures

Authors

  • Chan-Young Jeon,

    1. Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-Do, 200-702, Republic of Korea
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    • Chan-Young Jeon, Jae-Kwang Jin, and Young-Ho Koh contributed equally to this work.

  • Jae-Kwang Jin,

    1. Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Republic of Korea
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    • Chan-Young Jeon, Jae-Kwang Jin, and Young-Ho Koh contributed equally to this work.

  • Young-Ho Koh,

    1. Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Republic of Korea
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    • Chan-Young Jeon, Jae-Kwang Jin, and Young-Ho Koh contributed equally to this work.

  • Wook Chun,

    1. Department of Burn Surgery, Hangang Sacred Heart Hospital, Hallym University, Yongdeungpo-gu, 150-719, Seoul, Republic of Korea
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  • Ihn-Geun Choi,

    1. Department of Psychiatry, Hangang Sacred Heart Hospital, Hallym University, Yongdeungpo-gu, 150-719, Seoul, Republic of Korea
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  • Hyung-Joo Kown,

    1. Department of Microbiology, College of Medicine, Hallym University, Chuncheon, Kangwon-Do, 200-702, Republic of Korea
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  • Yong-Sun Kim,

    Corresponding author
    1. Ilsong Institute of Life Science, Hallym University, Anyang 431-060, Republic of Korea
    2. Department of Microbiology, College of Medicine, Hallym University, Chuncheon, Kangwon-Do, 200-702, Republic of Korea
    • Department of Microbiology, College of Medicine and Ilsong Institute of Life Science, Hallym University, Chuncheon, Kangwon-Do 200-702, Republic of Korea
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  • Jae-Bong Park

    Corresponding author
    1. Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-Do, 200-702, Republic of Korea
    • Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-Do 200-702, Republic of Korea
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Abstract

PC12 cells have been used as a model of sympathetic neurons. Nerve growth factor (NGF), basic fibroblast growth factor (bFGF), and cAMP induce neurite outgrowth from PC12 cells. cAMP induced a greater number of neurites than did NGF. In particular, we attempted to elucidate whether PC12 cell neurites, induced by several factors including NGF, bFGF, and cAMP, form synapses, and whether each neurite has presynaptic and postsynaptic properties. Using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), we observed that neurites are connected to each other. The connected regions presented dense core vesicles and a clathrin-coated membrane invagination. In addition, typical maker proteins for axon and dendrite were identified by an immuno-staining method. Tau-1, an axonal marker in neurons, was localized at a high concentration in the terminal tips of neurites from PC12 cells, which were connected to neurite processes containing MAP-2, a dendritic marker in neurons. Furthermore, neurites containing SV2 and synaptotagmin, markers of synaptic vesicles, were in contact with neurites harboring drebrin, a marker of the postsynaptic membrane, suggesting that neurites from PC12 cells induced by NGF, bFGF, and cAMP may form synapse-like structures. Tat-C3 toxin, a Rho inhibitor, augmented neurite outgrowth induced by NGF, bFGF, and cAMP. Tat-C3 toxin together with neurotrophins also exhibited synapse-like structures between neurites. However, it remains to be studied whether RhoA inhibition plays a role in the formation of synapse-like structures in PC12 cells. Synapse 64:765–772, 2010. © 2010 Wiley-Liss, Inc.

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