Glycine receptor internalization by protein kinases activation

Authors

  • Miguel ángel Velázquez-Flores,

    Corresponding author
    1. División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 México, D.F., México
    • División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México Apdo. Postal 70-253, 04,510 México, D.F., México
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  • Rocío Salceda

    1. División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 México, D.F., México
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Abstract

Although glycine-induced currents in the central nervous system have been proven to be modulated by protein kinases A (PKA) and C (PKC), the mechanism is not well understood. In order to better comprehend the mechanism involved in this phenomenon, we tested the PKA and PKC activation effect on the specific [3H]glycine and [3H]strychnine binding to postsynaptic glycine receptor (GlyR) in intact rat retina. The specific binding constituted about 20% of the total radioligand binding. Kinetic analysis of the specific binding exhibited a sigmoidal behavior with three glycine and two strychnine binding sites and affinities of 212 nM for [3H]glycine and 50 nM for [3H]strychnine. Specific radioligand binding was decreased (60–85%) by PKA and PKC activation, an effect that was blocked by specific kinases inhibitors, as well as by cytochalasin D. GlyR expressed in the plasma membrane decreased about 50% in response to kinases activation, which was consistent with an increase of the receptor in the microsomal fraction when PKA was activated. Moreover, immunoprecipitation studies indicated that these kinases lead to a time-dependent receptor phosphorylation. Our results suggest that in retina, GlyR is cross-regulated by G protein-coupled receptors, activating PKA and PKC. Synapse 2011. © 2011 Wiley-Liss, Inc.

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