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Keywords:

  • anorexia nervosa;
  • receptor trafficking;
  • anxiety;
  • mood;
  • stress;
  • food restriction;
  • hippocampus;
  • hyperactivity;
  • exercise;
  • tonic inhibition;
  • puberty;
  • adolescence;
  • eating disorder;
  • allopregnanolone;
  • pregnanolone;
  • progesterone;
  • THP;
  • hippocampus;
  • electron microscopic immunocytochemistry

Abstract

Activity-based anorexia (ABA) is an animal model for anorexia nervosa that has revealed genetic links to anxiety traits and neurochemical characteristics within the hypothalamus. However, few studies have used this animal model to investigate the biological basis for vulnerability of pubertal and adolescent females to ABA, even though the great majority of the anorexia nervosa cases are females exhibiting the first symptoms during puberty. GABAergic inhibition of the hippocampus strongly regulates anxiety as well as plasticity throughout life. We recently showed that the hippocampal CA1 of female mice undergo a dramatic change at puberty onset—from expressing virtually none of the nonsynaptic α4βδ GABAA receptors (GABARs) prepubertally to expressing these GABARs at ∼7% of the CA1 dendritic spine membranes at puberty onset. Furthermore, we showed that this change underlies the enhanced modulation of anxiety, neuronal excitability, and NMDA receptor-dependent synaptic plasticity in the hippocampus by the stress neurosteroid, THP (3α-OH-5α[β]-pregnan-20-one or [allo]pregnanolone). Here, we used quantitative electron microscopy to determine whether ABA induction in female rats during adolescence also elevates the expression of α4 and δ subunits of α4βδ GABARs, as was observed at puberty onset for mice. Our analysis revealed that rats also exhibit a rise of α4 and δ subunits of α4βδ GABARs at puberty onset, in that these subunits are detectable at ∼6% of the dendritic spine membranes of CA1 pyramidal cells at puberty onset (postnatal day 32–36; P32–36) but this drops to about 2% by P40–P44. The levels of α4 and δ subunits at the CA1 spines remained low following exposure of females to either of the two environmental factors needed to generate ABA—food restriction and access to a running wheel for 4 days—from P40 to P44. This pattern contrasted greatly from those of ABA animals, for which the two environmental factors were combined. Within the hippocampus of ABA animals, 12% of the spine profiles were labeled for α4, reflecting a sixfold increase, relative to hippocampi of age-matched (P44) control females (p < 0.005). Concurrently, 7% of the spine profiles were labeled for δ, reflecting a 130% increase from the control values of 3% (p = 0.01). No measurable change was detected for spine size. The observed magnitude of increase in the α4 and δ subunits at spines is sufficient to increase both tonic inhibition of hippocampus and anxiety during stress, thereby likely to exacerbate hyperactivity and weight loss. Synapse, 2012. © 2011 Wiley Periodicals, Inc.