Transient increases in dendritic spine density contribute to dentate gyrus long-term potentiation
Version of Record online: 28 FEB 2012
Copyright © 2012 Wiley Periodicals, Inc.
Volume 66, Issue 7, pages 661–664, July 2012
How to Cite
Wosiski-Kuhn, M. and Stranahan, A. M. (2012), Transient increases in dendritic spine density contribute to dentate gyrus long-term potentiation. Synapse, 66: 661–664. doi: 10.1002/syn.21545
- Issue online: 11 MAY 2012
- Version of Record online: 28 FEB 2012
- Accepted manuscript online: 8 FEB 2012 02:09AM EST
- Manuscript Received: 2 DEC 2011
- Manuscript Accepted: 26 JAN 2011
- Georgia Health Sciences University
- medial perforant path;
- synaptic plasticity;
- dentate gyrus
Dendritic spines are the primary sites for excitatory neurotransmission in the adult brain and exhibit changes in their number and morphology with experience. The relationship between spine formation and synaptic activity has been best characterized along the apical dendrites of pyramidal neurons in the hippocampal CA1 subfield. However, less is known about the structural mechanisms at the spine that mediate plasticity in other hippocampal subfields. The dentate gyrus is the predominant point of entry for synaptic input to the hippocampus, and dentate granule cells differ from CA1 pyramidal neurons in terms of their morphology and biophysical properties. In order to understand the structural mechanisms for plasticity in the dentate gyrus, we measured dendritic spine density in hippocampal slice preparations at different intervals following synaptic stimulation. We observed that transient increases in dendritic spine density are detectable 30 min after induction of long-term potentiation (LTP). By 60 min poststimulation, dendritic spine density has returned to basal levels. Both early LTP and enhancements in dendritic spine density could beblocked by destabilizing actin filaments, but not by inhibitors of transcription or protein synthesis. These results indicate that spine formation is a transient event that is required for dentate gyrus LTP. Synapse, 2012. © 2011 Wiley Periodicals, Inc.