P.G. and J.G.H. contributed equally to this work.
Hippocampal serotonin-1A receptor function in a mouse model of anxiety induced by long-term voluntary wheel running
Article first published online: 18 APR 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 67, Issue 10, pages 648–655, October 2013
How to Cite
Fuss, J., Vogt, M. A., Weber, K.-J., Burke, T. F., Gass, P. and Hensler, J. G. (2013), Hippocampal serotonin-1A receptor function in a mouse model of anxiety induced by long-term voluntary wheel running. Synapse, 67: 648–655. doi: 10.1002/syn.21666
- Issue published online: 23 AUG 2013
- Article first published online: 18 APR 2013
- Accepted manuscript online: 18 MAR 2013 02:53AM EST
- Manuscript Accepted: 9 MAR 2013
- Manuscript Received: 1 JAN 2013
- Deutsche Forschungsgemeinschaft . Grant Numbers: GA427/9-1 , SFB636-B3 (to P.G.)
- National Institute of Mental Health . Grant Number: MH 083300 (to J.G.H.)
- raphe nucleus;
We have recently demonstrated that, in C57/Bl6 mice, long-term voluntary wheel running is anxiogenic, and focal hippocampal irradiation prevents the increase in anxiety-like behaviors and neurobiological changes in the hippocampus induced by wheel running. Evidence supports a role of hippocampal 5-HT1A receptors in anxiety. Therefore, we investigated hippocampal binding and function of 5-HT1A receptors in this mouse model of anxiety. Four weeks of voluntary wheel running resulted in hippocampal subregion-specific changes in 5-HT1A receptor binding sites and function, as measured by autoradiography of [3H] 8-hydroxy-2-(di-n-propylamino)tetralin binding and agonist-stimulated binding of [35S]GTPγS to G proteins, respectively. In the dorsal CA1 region, 5-HT1A receptor binding and function were not altered by wheel running or irradiation. In the dorsal dentate gyrus and CA2/3 region, 5-HT1A receptor function was decreased by not only running but also irradiation. In the ventral pyramidal layer, wheel running resulted in a decrease of 5-HT1A receptor function, which was prevented by irradiation. Neither irradiation nor wheel running affected 5-HT1A receptors in medial prefrontal cortex or in the dorsal or median raphe nuclei. Our data indicate that downregulation of 5-HT1A receptor function in ventral pyramidal layer may play a role in anxiety-like behavior induced by wheel running. Synapse 67:648–655, 2013. © 2013 Wiley Periodicals, Inc.