Chao-Jian Pang and Lei Tong contributed equally to this work.
Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts
Article first published online: 2 MAY 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 67, Issue 10, pages 637–647, October 2013
How to Cite
Pang, C.-j., Tong, L., Ji, L.-l., Wang, Z.-y., Zhang, X., Gao, H., Jia, H., Zhang, L.-x. and Tong, X.-j. (2013), Synergistic effects of ultrashort wave and bone marrow stromal cells on nerve regeneration with acellular nerve allografts. Synapse, 67: 637–647. doi: 10.1002/syn.21669
- Issue published online: 23 AUG 2013
- Article first published online: 2 MAY 2013
- Accepted manuscript online: 30 MAR 2013 12:37AM EST
- Manuscript Accepted: 22 MAR 2013
- Manuscript Revised: 7 JAN 2013
- Manuscript Received: 16 OCT 2012
- Research Fund for Social Development of Science and Technology of Liaoning Province, China. Grant Number: 2010225029
- Project Grant of Science and Technology in Shenyang, China. Grant Number: F10-205-1-69
- National Natural Science Foundation of China. Grant Number: 81260193
- Natural Science Foundation of Ningxia of China. Grant Number: NZ12187
- ultrashort wave therapy;
- peripheral nerve regeneration;
- Schwann cells;
- bone marrow stromal cells
Acellular nerve allografts (ANA) possess bioactivity and neurite promoting factors in nerve tissue engineering. Previously we reported that low dose ultrashort wave (USW) radiation could enhance the rate and quality of peripheral nerve regeneration with ANA repairing sciatic nerve defects. Meanwhile, ANA implanted with bone marrow stromal cells (BMSCs) exhibited a similar result. Thus, it is interesting to know whether it might yield a synergistic effect when USW radiation is combined with BMSCs-laden ANA. Here we investigated the effectiveness of ANA seeded with BMSCs, combined with USW therapy on repairing peripheral nerve injuries. Adult male Wistar rats were randomly divided into four groups: Dulbecco's modified Eagle's medium (DMEM) control group, BMSCs-laden group, ultrashort wave (USW) group and BMSC + USW group. The regenerated nerves were assayed morphologically and functionally, and growth-promoting factors in the regenerated tissues following USW administration or BMSCs integration were also detected. The results indicated that the combination therapy caused much better beneficial effects evidenced by increased myelinated nerve fiber number, myelin sheath thickness, axon diameter, sciatic function index, nerve conduction velocity, and restoration rate of tibialis anterior wet weight. Moreover, the mRNA levels of brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) in the spinal cord and muscles were elevated significantly. In conclusion, we found a synergistic effect of USW radiation and BMSCs treatment on peripheral nerve regeneration, which may help establish novel strategies for repairing peripheral nerve defects. Synapse 67:637–647, 2013. © 2013 Wiley Periodicals, Inc.