Different pH-dependencies of the two synaptic adhesion molecules N-cadherin and cadherin-11 and the possible functional implication for long-term potentiation
Article first published online: 3 JUN 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 67, Issue 10, pages 705–715, October 2013
How to Cite
Baumgartner, W., Osmanagic, A., Gebhard, M., Kraemer, S. and Golenhofen, N. (2013), Different pH-dependencies of the two synaptic adhesion molecules N-cadherin and cadherin-11 and the possible functional implication for long-term potentiation. Synapse, 67: 705–715. doi: 10.1002/syn.21679
- Issue published online: 23 AUG 2013
- Article first published online: 3 JUN 2013
- Accepted manuscript online: 4 MAY 2013 12:26AM EST
- Manuscript Accepted: 17 APR 2013
- Manuscript Received: 27 NOV 2012
- Deutsche Forschungsgemeinschaft. Grant Numbers: GO 636/3-1, BA 2272/6-1
- synaptic plasticity;
- cell adhesion;
Ca2+-dependent adhesion molecules, cadherins, localised at synaptic sites are critically involved in long-term potentiation (LTP). N-cadherin is thought to promote LTP whereas cadherin-11 seems to counteract LTP. Since high synaptic activity is accompanied by local transient changes of the pH in the synaptic cleft, we studied whether the binding activity of cadherins is dependent on the pH and whether this might play a role during LTP. By atomic force microscopy (AFM) and laser tweezer experiments, we could show on the single molecule level as well as in a cell-based system that a decrease of the pH from 7.4 to 7.0 will result in a significant weakening of N-cadherin binding activity but in an increase of cadherin-11 binding. These differences in the pH dependencies of both molecules could be one explanation for their opposing roles during LTP. High-frequency stimulation will lead to a local acidosis in the synaptic cleft resulting in weakening of N-cadherin-mediated adhesion facilitating synaptic remodeling and LTP induction, whereas cadherin-11 bonds will be strengthened counteracting synaptic remodeling and LTP generation. Synapse 67:705–715, 2013. © 2013 Wiley Periodicals, Inc.