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Diminution of the NMDA receptor NR2B subunit in cortical and subcortical areas of WAG/Rij rats

Authors

  • Fariba Karimzadeh,

    1. Tehran University of Medical Sciences, Tehran, Iran
    2. Shefa Neuroscience Research Centre, Tehran, Iran
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  • Mansoureh Soleimani,

    1. Iran University of Medical Sciences, Tehran, Iran
    2. Cellular and Molecular Research Center and Department of anatomy, Tehran, Iran
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  • Mehdi Mehdizadeh,

    1. Iran University of Medical Sciences, Tehran, Iran
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  • Maryam Jafarian,

    1. Tehran University of Medical Sciences, Tehran, Iran
    2. Shefa Neuroscience Research Centre, Tehran, Iran
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  • Maliheh Mohamadpour,

    1. Shefa Neuroscience Research Centre, Tehran, Iran
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  • Hadi Kazemi,

    1. Department of Pediatric, Shahed University, Tehran, Iran
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  • Mohammad-Taghi Joghataei,

    Corresponding author
    1. Iran University of Medical Sciences, Tehran, Iran
    2. Cellular and Molecular Research Center and Department of anatomy, Tehran, Iran
    • Correspondence to: Mohammad-Taghi Joghataei, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran/Ali Gorji, Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Münster, Germany. E-mail: joghataei@iums.ac.ir/gorjial@uni-muenster.de

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  • Ali Gorji

    1. Shefa Neuroscience Research Centre, Tehran, Iran
    2. Institut für Physiologie I, Westfälische Wilhelms-Universität Münster, Germany
    3. Klinik und Poliklinik für Neurochirurgie, Westfälische Wilhelms-Universität Münster, Germany
    4. Department of Neurology, Westfälische Wilhelms-Universität Münster, Germany
    5. Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Germany
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ABSTRACT

Modulation of glutamatergic NMDA receptors affects the synchronization of spike discharges in in WAG/Rij rats, a valid genetic animal model of absence epilepsy. In this study, we describe the alteration of NR2B subunit of NMDA receptors expression in WAG/Rij rats in different somatosensory cortical layers and in hippocampal CA1 area. Experimental groups were divided into four groups of six rats of both WAG/Rij and Wistar strains with 2 and 6 months of age. The distribution of NR2B receptors was assessed by immunohistochemical staining in WAG/Rij and compared with age-matched Wistar rats. The expression of NR2B subunit was significantly decreased in different somatosensory cortical layers in 2- and 6-month-old WAG/Rij rats. In addition, the distribution of NR2B in hippocampal CA1 area was lower in 6-month-old WAG/Rij compared with age-matched Wistar rats. The reduction of NR2B receptors in different brain areas points to disturbance of glutamate receptors expression in cortical and subcortical areas in WAG/Rij rats. An altered subunit assembly of NMDA receptors may underlie cortical hyperexcitability in absence epilepsy. Synapse 67:839–846, 2013. © 2013 Wiley Periodicals, Inc.

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