The effects of nicotine and tobacco particulate matter on dopamine uptake in the rat brain
Article first published online: 30 SEP 2013
Copyright © 2013 Wiley Periodicals, Inc.
Volume 68, Issue 2, pages 45–60, February 2014
How to Cite
Danielson, K., Putt, F., Truman, P. and Kivell, B. M. (2014), The effects of nicotine and tobacco particulate matter on dopamine uptake in the rat brain. Synapse, 68: 45–60. doi: 10.1002/syn.21715
- Issue published online: 12 DEC 2013
- Article first published online: 30 SEP 2013
- Accepted manuscript online: 2 SEP 2013 10:43AM EST
- Manuscript Accepted: 28 AUG 2013
- Manuscript Received: 9 MAY 2013
- Neurological Foundation of New Zealand and the Wellington Medical Research Foundation, New Zealand
- dopamine transporter;
- norepinephrine transporter;
Cigarette smoking is the leading cause of preventable death worldwide. Recently, tobacco extracts have been shown to have a different pharmacological profile to nicotine alone and there is increasing evidence of a role for non-nicotinic components of cigarette smoke in smoking addiction. Nicotine is known to affect the uptake of dopamine in the brain of laboratory animals, but studies in the literature are often contradictory and little is known of the effects on non-nicotinic tobacco components on dopamine uptake. This study has examined the acute and chronic effects of nicotine and a tobacco extract (TPM) on dopamine uptake by the dopamine and norepinephrine transporters (DAT and NET) ex vivo using rotating disk electrode voltammetry, and quantified DAT and NET protein and mRNA expression in key brain regions. Nicotine (0.35 mg/kg) significantly decreased DAT function in the nucleus accumbens (NAc) at 30 min with no change in protein expression. This effect was sensitive to mecamylamine and DHβE but not MLA, indicating that it is dependent on α4 subunit containing nicotinic receptors. Furthermore, TPM, but not nicotine, increased DAT function in the dorsal striatum at 1 h in a nicotinic receptor independent manner with no change in DAT protein expression. At 1 h DAT mRNA in the ventral tegmental area was decreased by both acute and chronic TPM treatments. Synapse 68:45–60, 2014. © 2013 Wiley Periodicals, Inc.