These authors equally contributed to this study.
Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering
Article first published online: 4 DEC 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Tissue Engineering and Regenerative Medicine
How to Cite
Klopsch, C., Gäbel, R., Kaminski, A., Mark, P., Wang, W., Toelk, A., Delyagina, E., Kleiner, G., Koch, L., Chichkov, B., Mela, P., Jockenhoevel, S., Ma, N. and Steinhoff, G. (2012), Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering. J Tissue Eng Regen Med. doi: 10.1002/term.1657
- Article first published online: 4 DEC 2012
- Manuscript Accepted: 21 OCT 2012
- Manuscript Revised: 14 AUG 2012
- Manuscript Received: 2 APR 2012
- laser-induced forward transfer;
- cell printing;
- sprayed cell seeding;
- stem cell-plus-fibrin coating;
- intraoperative table-side tissue engineering;
- heart valve
At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies, spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs), umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on poly(ε-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun PCL tissue equivalents. Grafts were monitored in vitro under static and dynamic conditions in bioreactors. SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern, respectively. The engineered valves demonstrated immediate sufficient performance, complete cell coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular matrix deposition. SaBP revealed higher efficiency, with at least 12-fold shorter processing time than the applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell–scaffold distance. Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns. Copyright © 2012 John Wiley & Sons, Ltd.