Spray- and laser-assisted biomaterial processing for fast and efficient autologous cell-plus-matrix tissue engineering

Authors

  • Christian Klopsch,

    Corresponding author
    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
    • Correspondence to: Christian Klopsch, Department of Cardiac Surgery, Medical Faculty, University of Rostock, Schillingallee 35, 18057 Rostock, Germany. E-mail: christian.klopsch@med.uni-rostock.de

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    • These authors equally contributed to this study.

  • Ralf Gäbel,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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    • These authors equally contributed to this study.

  • Alexander Kaminski,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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  • Peter Mark,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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  • Weiwei Wang,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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    • Present address: BCRT and CBD, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Teltow, Germany.

  • Anita Toelk,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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  • Evgenya Delyagina,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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  • Gabriela Kleiner,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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  • Lothar Koch,

    1. Laser Zentrum Hannover, Germany
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  • Boris Chichkov,

    1. Laser Zentrum Hannover, Germany
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  • Petra Mela,

    1. Department of Tissue Engineering and Textile Implants, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Germany
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  • Stefan Jockenhoevel,

    1. Department of Tissue Engineering and Textile Implants, AME-Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Germany
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  • Nan Ma,

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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    • Present address: BCRT and CBD, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Teltow, Germany.

  • Gustav Steinhoff

    1. Reference and Translation Centre for Cardiac Stem Cell Therapy, University of Rostock, Germany
    2. Department of Cardiac Surgery, Medical Faculty, University of Rostock, Germany
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Abstract

At present, intensive investigation aims at the creation of optimal valvular prostheses. We introduced and tested the applicability and functionality of two advanced cell-plus-matrix seeding technologies, spray-assisted bioprocessing (SaBP) and laser-assisted bioprocessing (LaBP), for autologous tissue engineering (TE) of bioresorbable artificial grafts. For SaBP, human mesenchymal stem cells (HMSCs), umbilical cord vein endothelial cells (HUVECs) and fibrin were simultaneously spray-administered on poly(ε-caprolactone) (PCL) substrates. For LaBP, HUVECs and HMSCs were separately laser-printed in stripes, followed by fibrin sealing. Three-leaflet valves were manufactured following TE of electrospun PCL tissue equivalents. Grafts were monitored in vitro under static and dynamic conditions in bioreactors. SaBP and LaBP resulted in TE of grafts with homogeneous cell distribution and accurate cell pattern, respectively. The engineered valves demonstrated immediate sufficient performance, complete cell coating, proliferation, engraftment, HUVEC-mediated invasion, HMSC differentiation and extracellular matrix deposition. SaBP revealed higher efficiency, with at least 12-fold shorter processing time than the applied LaBP set-up. LaBP realized coating with higher cell density and minimal cell–scaffold distance. Fibrin and PCL stability remain issues for improvement. The introduced TE technologies resulted in complete valvular cell-plus-matrix coating, excellent engraftment and HMSCs differentiation. SaBP might have potential for intraoperative table-side TE considering the procedural duration and ease of implementation. LaBP might accelerate engraftment with precise patterns. Copyright © 2012 John Wiley & Sons, Ltd.

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