Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications
Article first published online: 11 SEP 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Tissue Engineering and Regenerative Medicine
How to Cite
Allori, A. C., Davidson, E. H., Reformat, D. D., Sailon, A. M., Freeman, J., Vaughan, A., Wootton, D., Clark, E., Ricci, J. L. and Warren, S. M. (2013), Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications. J Tissue Eng Regen Med. doi: 10.1002/term.1810
- Article first published online: 11 SEP 2013
- Manuscript Accepted: 22 JUL 2013
- Manuscript Revised: 20 JAN 2013
- Manuscript Received: 7 FEB 2012
- tissue engineering;
- lacunocanalicular system;
- cell culture;
- fluid shear stress
Bone lacunocanalicular fluid flow ensures chemotransportation and provides a mechanical stimulus to cells. Traditional static cell-culture methods are ill-suited to study the intricacies of bone biology because they ignore the three-dimensionality of meaningful cellular networks and the lacunocanalicular system; furthermore, reliance on diffusion alone for nutrient supply and waste product removal effectively limits scaffolds to 2–3 mm thickness. In this project, a flow-perfusion system was custom-designed to overcome these limitations: eight adaptable chambers housed cylindrical cell-seeded scaffolds measuring 12 or 24 mm in diameter and 1–10 mm in thickness. The porous scaffolds were manufactured using a three-dimensional (3D) periodic microprinting process and were composed of hydroxyapatite/tricalcium phosphate with variable thicknesses, strut sizes, pore sizes and structural configurations. A multi-channel peristaltic pump drew medium from parallel reservoirs and perfused it through each scaffold at a programmable rate. Hermetically sealed valves permitted sampling or replacement of medium. A gas-permeable membrane allowed for gas exchange. Tubing was selected to withstand continuous perfusion for > 2 months without leakage. Computational modelling was performed to assess the adequacy of oxygen supply and the range of fluid shear stress in the bioreactor–scaffold system, using 12 × 6 mm scaffolds, and these models suggested scaffold design modifications that improved oxygen delivery while enhancing physiological shear stress. This system may prove useful in studying complex 3D bone biology and in developing strategies for engineering thick 3D bone constructs. Copyright © 2013 John Wiley & Sons, Ltd.