Abdominal wall reconstruction by a regionally distinct biocomposite of extracellular matrix digest and a biodegradable elastomer

Authors

  • Keisuke Takanari,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Surgery, Pittsburgh, PA, USA
    Search for more papers by this author
    • Present address: Nagoya University, Department of Plastic and Reconstructive Surgery, Nagoya, Aichi, Japan.

    • #

      Both authors contributed equally to this work.

  • Yi Hong,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Surgery, Pittsburgh, PA, USA
    Search for more papers by this author
    • Present address: University of Texas at Arlington, Department of Bioengineering, Arlington, TX, USA.

    • #

      Both authors contributed equally to this work.

  • Ryotaro Hashizume,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Surgery, Pittsburgh, PA, USA
    Search for more papers by this author
    • §

      Present address: Mie University Graduate School of Medicine, Department of Pathology and Matrix Biology, Tsu, Mie, Japan.

  • Alexander Huber,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Surgery, Pittsburgh, PA, USA
    Search for more papers by this author
  • Nicholas J. Amoroso,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Bioengineering, Pittsburgh, PA, USA
    Search for more papers by this author
  • Antonio D'Amore,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Bioengineering, Pittsburgh, PA, USA
    3. RiMED Foundation, Palermo, Italy
    4. DICGIM University of Palermo, Palermo, Italy
    Search for more papers by this author
  • Stephen F. Badylak,

    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Surgery, Pittsburgh, PA, USA
    3. University of Pittsburgh, Department of Bioengineering, Pittsburgh, PA, USA
    Search for more papers by this author
  • William R. Wagner

    Corresponding author
    1. University of Pittsburgh, McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA
    2. University of Pittsburgh, Department of Surgery, Pittsburgh, PA, USA
    3. University of Pittsburgh, Department of Bioengineering, Pittsburgh, PA, USA
    4. University of Pittsburgh, Department of Chemical Engineering, Pittsburgh, PA, USA
    • Correspondence to: W. R. Wagner, McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA.

      E-mail: wagnerwr@upmc.edu

    Search for more papers by this author

Abstract

Current extracellular matrix (ECM) derived scaffolds offer promising regenerative responses in many settings, however in some applications there may be a desire for more robust and long lasting mechanical properties. A biohybrid composite material that offers both strength and bioactivity for optimal healing towards native tissue behavior may offer a solution to this problem. A regionally distinct biocomposite scaffold composed of a biodegradable elastomer (poly(ester urethane)urea) and porcine dermal ECM gel was generated to meet this need by a concurrent polymer electrospinning/ECM gel electrospraying technique where the electrosprayed component was varied temporally during the processing. A sandwich structure was achieved with polymer fiber rich upper and lower layers for structural support and an ECM-rich inner layer to encourage cell ingrowth. Increasing the upper and lower layer fiber content predictably increased tensile strength. In a rat full thickness abdominal wall defect model, the sandwich scaffold design maintained its thickness whereas control biohybrid scaffolds lacking the upper and lower fiber-rich regions failed at 8 weeks. Sandwich scaffold implants also showed higher collagen content 4 and 8 weeks after implantation, exhibited an increased M2 macrophage phenotype response at later times and developed biaxial mechanical properties better approximating native tissue. By employing a processing approach that creates a sheet-form scaffold with regionally distinct zones, it was possible to improve biological outcomes in body wall repair and provide the means for further tuning scaffold mechanical parameters when targeting other applications. Copyright © 2013 John Wiley & Sons, Ltd.

Ancillary