Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds
Version of Record online: 8 NOV 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Tissue Engineering and Regenerative Medicine
How to Cite
Leferink, A. M., Hendrikson, W. J., Rouwkema, J., Karperien, M., van Blitterswijk, C. A. and Moroni, L. (2013), Increased cell seeding efficiency in bioplotted three-dimensional PEOT/PBT scaffolds. J Tissue Eng Regen Med. doi: 10.1002/term.1842
- Version of Record online: 8 NOV 2013
- Manuscript Accepted: 13 SEP 2013
- Manuscript Revised: 20 AUG 2013
- Manuscript Received: 25 FEB 2013
- cell seeding efficiency;
- mesenchymal stromal cells;
- rapid prototyping
In regenerative medicine studies, cell seeding efficiency is not only optimized by changing the chemistry of the biomaterials used as cell culture substrates, but also by altering scaffold geometry, culture and seeding conditions. In this study, the importance of seeding parameters, such as initial cell number, seeding volume, seeding concentration and seeding condition is shown. Human mesenchymal stem cells (hMSCs) were seeded into cylindrically shaped 4 × 3 mm polymeric scaffolds, fabricated by fused deposition modelling. The initial cell number ranged from 5 × 104 to 8 × 105 cells, in volumes varying from 50 µl to 400 µl. To study the effect of seeding conditions, a dynamic system, by means of an agitation plate, was compared with static culture for both scaffolds placed in a well plate or in a confined agarose moulded well. Cell seeding efficiency decreased when seeded with high initial cell numbers, whereas 2 × 105 cells seemed to be an optimal initial cell number in the scaffolds used here. The influence of seeding volume was shown to be dependent on the initial cell number used. By optimizing seeding parameters for each specific culture system, a more efficient use of donor cells can be achieved. Copyright © 2013 John Wiley & Sons, Ltd.