Adipose- and bone marrow-derived mesenchymal stem cells display different osteogenic differentiation patterns in 3D bioactive glass-based scaffolds

Authors

  • Subha N. Rath,

    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
    2. Department of Biomedical Engineering, Indian Institute of Technology, Hyderabad, India
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  • Patcharakamon Nooeaid,

    1. Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Andreas Arkudas,

    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Justus P. Beier,

    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Leonie A. Strobel,

    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
    2. Department of Hand, Plastic and Reconstructive Surgery – Burns Centre, BG Trauma Centre Ludwigshafen and Department of Plastic Surgery, University of Heidelberg, Germany
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  • Andreas Brandl,

    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Judith A. Roether,

    1. Institute of Polymer Materials, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Raymund E. Horch,

    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Aldo R. Boccaccini,

    1. Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen–Nürnberg, Erlangen, Germany
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  • Ulrich Kneser

    Corresponding author
    1. Department of Plastic and Hand Surgery, Laboratory for Tissue Engineering and Regenerative Medicine, Nikolaus Fiebiger Zentrum, University of Erlangen–Nürnberg, Erlangen, Germany
    2. Department of Hand, Plastic and Reconstructive Surgery – Burns Centre, BG Trauma Centre Ludwigshafen and Department of Plastic Surgery, University of Heidelberg, Germany
    • Correspondence to: Ulrich Kneser, Department of Hand, Plastic and Reconstructive Surgery – Burns Centre, BG Trauma Centre Ludwigshafen, University of Heidelberg, Ludwig-Guttmann-Strasse 13, 67071 Ludwigshafen, Germany. E-mail: Ulrich.kneser@bgu-ludwigshafen.de

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Abstract

Mesenchymal stem cells can be isolated from a variety of different sources, each having their own peculiar merits and drawbacks. Although a number of studies have been conducted comparing these stem cells for their osteo-differentiation ability, these are mostly done in culture plastics. We have selected stem cells from either adipose tissue (ADSCs) or bone marrow (BMSCs) and studied their differentiation ability in highly porous three-dimensional (3D) 45S5 Bioglass®-based scaffolds. Equal numbers of cells were seeded onto 5 × 5 × 4 mm3 scaffolds and cultured in vitro, with or without osteo-induction medium. After 2 and 4 weeks, the cell–scaffold constructs were analysed for cell number, cell spreading, viability, alkaline phosphatase activity and osteogenic gene expression. The scaffolds with ADSCs displayed osteo-differentiation even without osteo-induction medium; however, with osteo-induction medium osteogenic differentiation was further increased. In contrast, the scaffolds with BMSCs showed no osteo-differentiation without osteo-induction medium; after application of osteo-induction medium, osteo-differentiation was confirmed, although lower than in scaffolds with ADSCs. In general, stem cells in 3D bioactive glass scaffolds differentiated better than cells in culture plastics with respect to their ALP content and osteogenic gene expression. In summary, 45S5 Bioglass-based scaffolds seeded with ADSCs are well-suited for possible bone tissue-engineering applications. Induction of osteogenic differentiation appears unnecessary prior to implantation in this specific setting. Copyright © 2013 John Wiley & Sons, Ltd.

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