Research Article

Using swept-source optical coherence tomography to monitor the formation of neo-epidermis in tissue-engineered skin

  1. Louise E. Smith1,
  2. Marco Bonesi1,†,
  3. Rod Smallwood2,
  4. Stephen J. Matcher1,
  5. Sheila MacNeil1,*

Article first published online: 17 NOV 2010

DOI: 10.1002/term.281

Issue

Journal of Tissue Engineering and Regenerative Medicine

Journal of Tissue Engineering and Regenerative Medicine

Volume 4, Issue 8, pages 652–658, December 2010

Additional Information

How to Cite

Smith, L. E., Bonesi, M., Smallwood, R., Matcher, S. J. and MacNeil, S. (2010), Using swept-source optical coherence tomography to monitor the formation of neo-epidermis in tissue-engineered skin. Journal of Tissue Engineering and Regenerative Medicine, 4: 652–658. doi: 10.1002/term.281

Author Information

  1. 1

    Tissue Engineering Group, Kroto Research Institute, University of Sheffield, UK

  2. 2

    Department of Computer Science, 211 Portobello, Sheffield, UK

  1. †

    Now at the Medical University of Vienna, Centre for Biomedical Engineering and Physics, Divison of Medical Physics, Wäringerstrasse 13, 1090 Vienna, Austria.

*Tissue Engineering Group, Kroto Research Institute, North Campus, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.

Publication History

  1. Issue published online: 17 NOV 2010
  2. Article first published online: 17 NOV 2010
  3. Manuscript Accepted: 25 FEB 2010
  4. Manuscript Received: 15 OCT 2009

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Keywords:

  • non-invasive imaging;
  • optical coherence tomography;
  • tissue-engineered skin;
  • re-epithelialization;
  • acellular dermis;
  • collagen gels

Abstract

There is an increasing need for a robust, simple to use, non-invasive imaging technology to follow tissue-engineered constructs as they develop. Our aim was to evaluate the use of swept-source optical coherence tomography (SS-OCT) to image tissue-engineered skin as it developed over several weeks. Tissue-engineered skin was produced using both de-epithelialized acellular dermis (DED) and amorphous collagen gels. In both cases the epidermis could be readily distinguished from the neodermis, based on a comparison with standard destructive histology of samples. Constructs produced with DED showed more epidermal/dermal maturation than those produced using collagen. The development of tissue-engineered skin based on DED was accurately monitored with SS-OCT over 3 weeks and confirmed with conventional histology. Copyright © 2010 John Wiley & Sons, Ltd.

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