These authors contributed equally to the contents of this paper.
Advanced maturation by electrical stimulation: Differences in response between C2C12 and primary muscle progenitor cells
Version of Record online: 9 DEC 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Journal of Tissue Engineering and Regenerative Medicine
Volume 5, Issue 7, pages 529–539, July 2011
How to Cite
Langelaan, M. L. P., Boonen, K. J. M., Rosaria-Chak, K. Y., van der Schaft, D. W. J., Post, M. J. and Baaijens, F. P. T. (2011), Advanced maturation by electrical stimulation: Differences in response between C2C12 and primary muscle progenitor cells. J Tissue Eng Regen Med, 5: 529–539. doi: 10.1002/term.345
- Issue online: 22 JUN 2011
- Version of Record online: 9 DEC 2010
- Manuscript Accepted: 8 JUL 2010
- Manuscript Received: 9 MAR 2010
- skeletal muscle tissue engineering;
- biophysical stimulation;
- muscle progenitor cells;
- cell line vs. primary cell source;
- sarcomere assembly
Skeletal muscle tissue engineering still does not result in the desired functional properties and texture as preferred for regenerative medicine and meat production applications. Electrical stimulation has been appropriately used as a tool to advance muscle cell maturation in vitro, thereby simulating nerve stimulation, as part of the muscle cell niche in vivo. We first investigated the effects of electrical stimulation protocols in two-dimensional (2D) monolayers of C2C12 and translated these protocols to a three-dimensional (3D) model system, based on a collagen type I/Matrigel™ hydrogel. More importantly, we addressed the ongoing debate of the translation of results found in cell lines (C2C12) to a primary cell source [muscle progenitor cells (MPCs)] in our 3D system. Striking differences in maturation level were found between the different cell sources. Constructs with MPCs were much more mature than C2C12 constructs, based on developed cross-striations and expression levels of mature myosin heavy chain (MHC) isoforms. Overall, electrical stimulation, when optimally timed, accelerated sarcomere assembly in both 2D and 3D. In addition, MPC constructs were more susceptible to the electrical stimulus, resulting in a shift of MHC expression to slower isoforms. Copyright © 2010 John Wiley & Sons, Ltd.