Bone morphogenetic protein-2 in biodegradable gelatin and β-tricalcium phosphate sponges enhances the in vivo bone-forming capability of bone marrow mesenchymal stem cells
Article first published online: 5 MAY 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Tissue Engineering and Regenerative Medicine
Volume 6, Issue 4, pages 253–260, April 2012
How to Cite
Tadokoro, M., Matsushima, A., Kotobuki, N., Hirose, M., Kimura, Y., Tabata, Y., Hattori, K. and Ohgushi, H. (2012), Bone morphogenetic protein-2 in biodegradable gelatin and β-tricalcium phosphate sponges enhances the in vivo bone-forming capability of bone marrow mesenchymal stem cells. J Tissue Eng Regen Med, 6: 253–260. doi: 10.1002/term.427
- Issue published online: 2 APR 2012
- Article first published online: 5 MAY 2011
- Manuscript Accepted: 13 MAR 2011
- Manuscript Received: 21 JUL 2010
- bone morphogenetic protein-2 (BMP-2);
- controlled drug release;
- mesenchymal stem cell;
Bone marrow mesenchymal stem cells (MSCs) have been used for bone tissue engineering due to their osteogenic differentiation capability, but their application is controversial. To enhance their capability, we prepared biodegradable gelatin sponges incorporating β-tricalcium phosphate ceramics (GT sponge), which has been shown to possess excellent controlled drug-release properties. The GT sponge was used as a carrier for both rat MSCs and bone morphogenetic protein-2 (BMP-2) and osteogenic differentiation was assessed by subcutaneous implantation of four different kinds of implants, i.e. GT-alone, MSC–GT composites, BMP–GT composites and BMP–GT composites supplemented with MSCs (BMP–MSC–GT) in rats. Two weeks after implantation, histological sections showed new bone formation in the peripheral parts of the BMP–GT and in almost the total volume of the BMP–MSC–GT implants. After 4 weeks, histology as well as microCT analyses demonstrated extensive bone formation in BMP–MSC–GT implants. Gene expression and biochemical analyses of both alkaline phosphatase and bone-specific osteocalcin confirmed the histological findings. These results indicate that the combination of MSCs, GT and BMP synergistically enhances osteogenic capability and provides a rational basis for their clinical application in bone reconstruction. Copyright © 2011 John Wiley & Sons, Ltd.