Human mesenchymal stem cells as a stable source of VEGF-producing cells

Authors

  • Harumi Kagiwada,

    1. Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan
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  • Tetsuya Yashiki,

    1. Dainippon Sumitomo Pharma Co. Ltd, 2-6-8 Doshomachi, Chuo-ku, Osaka 541-0045, Japan
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  • Akira Ohshima,

    1. Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan
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  • Mika Tadokoro,

    1. Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan
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  • Noritoshi Nagaya,

    1. Department of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan
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  • Hajime Ohgushi

    Corresponding author
    1. Tissue Engineering Research Group, Research Institute for Cell Engineering (RICE), National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974, Japan
    • 3-11-46 Nakoji, Amagasaki, Hyogo 661-0974; Japan.
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Abstract

Vascular endothelial growth factor (VEGF) is a positive regulator and plays a crucial role in angiogenesis. We demonstrate that VEGF was highly expressed in cultures of human bone marrow-derived mesenchymal stem cells (hMSCs) and the high expression level was maintained during prolonged culture periods (checked up to passage 10). We also confirmed that in vivo hMSCs engrafted into immunodeficient mice could survive and secreted human VEGF. These findings suggest that implantation of hMSCs is a practical means as a source of VEGF production and might be effective in neoangiogenesis. Copyright © 2008 John Wiley & Sons, Ltd.

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