Ultrastructural effects of DDT, DDD, and DDE on neural cells of the chicken embryo model
Article first published online: 11 MAY 2007
Copyright © 2007 Wiley Periodicals, Inc.
Volume 22, Issue 3, pages 328–336, June 2007
How to Cite
Bornman, M. S., Pretorius, E., Marx, J., Smit, E. and van der Merwe, C. F. (2007), Ultrastructural effects of DDT, DDD, and DDE on neural cells of the chicken embryo model. Environ. Toxicol., 22: 328–336. doi: 10.1002/tox.20261
- Issue published online: 11 MAY 2007
- Article first published online: 11 MAY 2007
- Manuscript Accepted: 26 DEC 2006
- Manuscript Received: 11 DEC 2006
- National Research Foundation. Grant Numbers: FA2004033100004, FA2004042900094
- Water Research Commission. Grant Number: K8/639
- electron microscopy
Human exposure to environmental compounds with estrogenic activity and the potential effects on human health is the subject of ongoing scientific debates. Their potential effects raise concern regarding neurological development after prenatal exposure. Central to this debate is the pesticide 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT). Although it has apparent low acute toxicity in mammals, DDT has a long residual persistence and laboratory research has indicated that it acts on the CNS by interfering with Na+/K+ pump mechanism of the neuronal membranes, causing disruption in Ca2+ homeostasis. Potentially this may lead to both apoptosis and necrosis. The present study investigates the effects of DDT and two of its metabolites DDD and DDE on the ultramorphology of neural cells, using a previously published chicken embryo model. Results indicate cellular swelling, budding, and increased membrane permeability for all three chemicals, accompanied by karyolysis in the DDE group (typical features of oncosis). These results support the finding of other researchers as well as the concerns of the WHO that DDT and its metabolites may cause neurotoxicity after prenatal exposure. © 2007 Wiley Periodicals, Inc. Environ Toxicol 22: 328–336, 2007.