Toxicity of the antimicrobial compound triclosan and formation of the metabolite methyl-triclosan in estuarine systems
Article first published online: 23 JAN 2008
Copyright © 2008 Wiley Periodicals, Inc.
Volume 23, Issue 2, pages 224–232, April 2008
How to Cite
DeLorenzo, M. E., Keller, J. M., Arthur, C. D., Finnegan, M. C., Harper, H. E., Winder, V. L. and Zdankiewicz, D. L. (2008), Toxicity of the antimicrobial compound triclosan and formation of the metabolite methyl-triclosan in estuarine systems. Environ. Toxicol., 23: 224–232. doi: 10.1002/tox.20327
- Issue published online: 11 MAR 2008
- Article first published online: 23 JAN 2008
- Manuscript Accepted: 4 AUG 2007
- Manuscript Revised: 25 JUL 2007
- Manuscript Received: 7 MAY 2007
Triclosan, a commonly used antimicrobial compound, has been measured in aquatic systems worldwide. This study exposed marine species to triclosan to examine effects primarily on survival and to investigate the formation of the degradation product, methyl-triclosan, in the estuarine environment. Acute toxicity was assessed using the bacterium Vibrio fischeri, the phytoplankton species Dunaliella tertiolecta, and three life stages of the grass shrimp Palaemonetes pugio. P. pugio larvae were more sensitive to triclosan than adult shrimp or embryos. Acute aqueous toxicity values (96 h LC50) were 305 μg/L for adult shrimp, 154 μg/L for larvae, and 651 μg/L for embryos. The presence of sediment decreased triclosan toxicity in adult shrimp (24 h LC50s were 620 μg/L with sediment, and 482 μg/L without sediment). The bacterium was more sensitive to triclosan than the grass shrimp, with a 15 min aqueous IC50 value of 53 μg/L and a 15 min spiked sediment IC50 value of 616 μg/kg. The phytoplankton species was the most sensitive species tested, with a 96 h EC50 value of 3.55 μg/L. Adult grass shrimp were found to accumulate methyl-triclosan after a 14-day exposure to 100 μg/L triclosan, indicating formation of this metabolite in a seawater environment and its potential to bioaccumulate in higher organisms. Triclosan was detected in limited surface water sampling of Charleston Harbor, SC at a maximum concentration of 0.001 μg/L, substantially lower than the determined toxicity values. These findings suggest triclosan poses low acute toxicity risk to estuarine organisms; however, the potential for chronic, sublethal, and metabolite effects should be investigated. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2008.